The most abundant lysosomal membrane proteins are lysosomal-associated membrane protein-1 (LAMP-1, CD107a), LAMP-2 (CD 107b), and LAMP-3 (CD63
BAT for wheat was performed using two flow cytometric procedures based, respectively, on the expression of CD63
(Becton Dickinson-BD Biosciences, San Jose, CA) and CD203c (Beckman Coulter, Fullerton, CA) on the cell surface of basophils activated in vitro by exposure to wheat in double dilution.
Researchers then apply fluorescently labeled antibodies that specifically target two protein biomarkers for pancreatic cancer: glypican-1 and CD63
. If these biomarkers are present, brightly colored circles where the antibodies bind can be seen under a microscope, indicating a positive result.
Western blotting was used to assess the exosome biomarkers CD63
and flotillin as well as the negative control marker calreticulin (Figure 1B).
This study has been a natural continuation of our previously published research concerning the significance and the usefulness of GLUT-1, CD9, and CD63
To determine the effects of these culture conditions on our iPSC-MSCs, we examined the mRNA expressions of biomarkers used for the optimization including CD63
, TSG6, IL1[micro], and STC1, as well as those of factors related to immunosuppressive effects of MSCs or MSC EVs including IDO , iNOS , IL10, and TGF[beta]  by qRT-PCR in iPSC-MSCs cultured for 0, 6, 24, or 48 hours in the CDPF medium.
reported elevated total Annexin V EV, platelet EV (CD63
, P-selectin-exposing), and endothelial EV (CD62, E-selectin-exposing) in 27 young healthy women compared to men while at rest.
Some members of the tetraspanin family, such as CD63
, CD81, and CD9, are enriched in exosomes and have been recognized as exosome markers .
The exosomes-associated surface markers include Alix, TSG101, tetraspanins (CD9, CD63
, and CD81), flotillin 1, cell adhesion molecules (CAM), and integrins .
However, other evidence suggests that TNF signalling may enhance HSC function [38, 39]; (iiii) TIMP-1, through receptor (CD63
) binding, has a role in multiple biological processes, including inflammation and immune regulation.
Anaphylactic degranulation results in the fusion of the main histamine-containing granules with the plasma membrane, releasing the entire contents of granules to the extracellular space and exposing CD63
on the surface of basophils .
Two antigens (CD9 and CD63
) are widely used in EV research (21).