Effects of safflower extract and aceglutamide injection on expressions of CD62P
and CRP in patients with coronary heart disease of blood stasis type.
Moreover, they demonstrated that [epsilon]-PLL did not significantly affect the quality of the PCs except for increased CD62P
, also known as P-selectin or granule membrane protein 140 (GMP 140), is a glycoprotein of 140 kDa present in alpha (a)-granules of resting platelets and is translocated to the plasma membrane after activation.
Other mechanisms described in the literature include increased activation of Glycoprotein IIb/IIIa, CD41a, CD42b, CD62P
and CD6 (3,2,6) prothrombin fragment F1+ 2, thrombin-antithrombin III complex and fibrinopeptide A.
P-Selectin, also known as CD62P
, granule membrane protein (GMP) 140 or platelet activationdependent granule-external membrane(PADGEM) is a glycoprotein of 140 kDa.
Platelet activation was assessed by measuring changes in platelet surface expression of CD40L and CD62P
and changes in the number of platelet-leukocyte conjugates relative to the baseline values of each exposure.
Fifty-four people diagnosed with CFS or fibromyalgia and 23 controls were given five tests to evaluate their blood: fibrinogen (FIB), prothrombin fragment 1 + 2 (F1 + 2), thrombin/antithrombin complexes (T/AT), soluble fibrin monomer (SFM), and platelet activation by flow cytometry (PA) using CD62P
(P-selectin)-FITC antibody (BD, USA) was added and incubated at room temperature for 20 min before resuspension with 1 ml of phosphate-buffered saline (PBS) and tested by flow cytometer.
Activation of platelets with ADP resulted in an increase in the number of CD63 and CD62p
positive platelets with a p value of 0.
Results for CD62P
and CD154 were expressed as percentage of positive events and MFI gated on [CD61.
Effect of glycoprotein Ilb/Illa inhibitors on CD62p
expression, platelet aggregates, and microparticles in vitro.
FITC anti-human CD62P
was purchased from BD Biosciences Pharmingen, and peroxidase-conjugated goat anti-mouse IgG was purchased from Chemicon.