In addition, targeted phenotyping was limited to detection of the surface marker for CD62E
IGFBP-1: Insulin-like growth factor-binding protein 1 IGFBP-2: Insulin-like growth factor-binding protein 2 BDNF: Brain-derived neurotrophic factor L-Selectin: CD62L E-Selectin: CD62E
ICAM-1: Intercellular adhesion molecule 1 PECAM: Platelet endothelial cell adhesion molecule VCAM-1: Vascular cell adhesion protein 1 MIP-1d: Chemokine (C-C motif) ligand 15 (CCL15) MIP-3b: Macrophage inflammatory protein-3 (CCL19) Eotaxin-1: C-C motif chemokine 11 (CCL11) Eotaxin-2: Chemokine (C-C motif) ligand 24 (CCL24) BLC: B lymphocyte chemoattractant.
Activated T cells are known to enter the skin via inflamed blood vessels, by binding via selectin ligands to CD62P (P-selectin) and CD62E
(E-selectin) expressed by endothelial cells .
Bone marrow ECs express E-selectin (CD62E
) constitutively, whereas other types of ECs produce and express E-selectin only upon inflammatory activation.
After four hours of low-tide MV, plasma EMP levels were significantly increased in both the C57BL/6 and TLR4KO mice (P < 0.01 for CD144, P < 0.01 for CD62E
, P < 0.01 for CD31, P < 0.05 for CD51, and P < 0.05 for CD54).
Subsequently, we looked for CD146, CD34, CD62e
, and CD133 expression in other 2D fluorescence plots from R2.
[sup] Another study specified that only those activated EMPs (CD62E
positive) but not the apoptotic EMPs could predict cardiovascular events in 300 patients with a recent stroke.
For the detection of E-selectin, cells were fixed with 0.1% glutaraldehyde in PBS and then incubated with 300 ng/ml CD62E
mouse anti human antibody (R&D Systems, Minneapolis, USA) and horseradish peroxidase-linked sheep anti mouse IgG (GE Healthcare, Waukesha, USA).
The purity of the PMNS was determined to exceed 95% by Giemsa Staining Cells (1 x [10.sup.6] cells/tube) which were incubated with a rabbit polyclonal antibody to (CD62E
(ab18981; Abcam PIC332, Cambridge, UK) or control.
Furthermore, endothelial cell adhesion molecules are highly expressed after allotransplantation especially in GVHD-affected tissue, and soluble E-selectin (CD62E
) serum levels are increased during acute GVHD whereas levels of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1) are only increased in chronic GVHD [6-8].
Immunostaining for CD31 and CD62E
in ECs demonstrated that the cells maintained their phenotypes.
) is surface glycoprotein molecule involved in adhesion of circulating leukocyte in activated endothelium and plays an important role in inflammation process.