Considering higher positive expression of [CD19.sup.+], [CD20.sup.[+], CD79a.sup.+], [CD5.sup.+], and [CD43.sup.+] in MCL [41, 42], as well as the sizes of nanoparticles in our experiments, thus CD20 and CD5 antigens will be chosen as the target spots of specific nanoprobes.
If CD20 and CD5 nanoprobes are hybridized in situ with MCL, CD20, and CD5 antibodies should be in response to CD20 and CD5 antigens on the cytomembrane of MCL according to the interaction rule of antigens with their specific antibodies.
Based on specific antigen-antibody recognition, the expression levels of CD20 and/or CD5 antigens on Jeko-1 cell surface in this experiment can be adjusted by means of feeding different doses of CD20 and CD5 antibodies in cell culture to consume corresponding CD20 and/or CD5 antigens on Jeko-1 cells.
It is well known that CD20 and CD5 antigens are usually located on the cytomembrane of Jeko-1 cells.
Thus, the multicolored fluorescence spots in size in Figure 9 might be determined by the distribution of Jeko-1 cells in the mouse models, which is due to higher positive expression of CD20 and CD5 antigens in MCL.