We completed the first extension of the CD5
development during the 2017 winter season and are planning a second extension to add ten more well slots, for a total of forty-three wells.
The monoclonal antibodies (MoAb) used for diagnosis of AL were: for B-lineage: CD19, CD22, CD20, CD10, for T-lineage: CD2, CD3, CD5
, CD7, CD4, CD8, for myelo-monocytic lineage: CD13, CD33, CD11c, CD64, CD14, other cells surface markers: CD34, CD117, HLA-DR and intracellular MoAb: CD79a, CD3 and MPO.
The results were as follows: Flow cytometric immunophenotyping identifies a phenotypically distinct population of cells with low light scatter properties that express CD19, low density CD20, CD5
, and CD45 and display Kappa immunoglobulin light chain restriction.
3) According to published data, 80% to 92% of CLLs stain positive for CD5
, (4, 5) but the exact percentage of SLL that is positive for CD5
is not known.
Flow cytometry of peripheral blood revealed an expression of CD3, CD4, CD5
, TCR[alpha][beta] and CD2 with co-expression of CD25.
T-cell phenotype reaction was observed with LCA (CD45), CD3, CD5
and CD45RO (UCHL-1) in the mature lymphoid cells (Figure-2A, 2B).
Immunohistochemical examination of the specimen was positive for cytokeratin (CK)18, CK19, CK5/6 and CD5
and CD117, and negative for CK7, TTF1, CD5
6 and CD20 markers (Figure 4).
Immunohistochemistry stains were positive for CD20, CD5
, CD43, Bcl-2, and cyclin D1.
El LCM se caracteriza por una proliferacion monomorfica de linfocitos de tamano pequeno a intermedio, con nucleos irregulares que coexpresan CD5
es una glicoproteina transmembrana que se expresa en celulas T normales y en un grupo de celulas B neoplasicas (12).
In lymphoma CD20 and CD79a (pan B-cell markers), CD3 and CD5
(pan T-cell markers), CD15 and CD30 (Hodgkin lymphoma marker), CD10, CD23 and EMA were applied.
Some lymphoproliferative disorders like CD23 negative chronic lymphocytic leukemia's, CD5
negative mantle cell lymphomas, CD5
positive follicular lymphomas, CD10 negative follicular lymphomas and CD5
positive splenic marginal zone lymphomas also exist and should always be considered in differential diagnosis in appropriate cases.