PSC are anchored within the niche by expression of the surface marker CD49f
or integrin a6, which, when paired with either [beta]1 or [beta]4, can form an integrin receptor that binds to its ligand laminin, a protein found in its basement membrane and extracellular matrix (ECM) (53,57).
Protein Name CD name Heterodimer family [alpha]4 CD49d [alpha]4/[beta]1 [alpha]4/[beta]7 [alpha]6 CD49f
[alpha]6/[beta]1 Integrins [alpha]h, LFA-1 CD11a [alpha]L/[beta]2 [alpha]M, Mac-1 CD11b [alpha]M/[beta]1 [beta]1 CD29 [alpha]4/[beta]1 [alpha]6/[beta]1 [beta]2 CD18 [alpha]L/[beta]2 VLA-4 CD49d/CD29 [alpha]4/[beta]1 fIntegrin VLA-6 CD49f/CD29 [alpha]6/[beta]1 dimers LPAM-1 -- [alpha]4/[beta]7 E-selectin CD62E -- Selectins L-selectin CD62L -- P-selectin CD62P -- pMIC2?
HeLa MonoLayer was cultured for 48 hours, and HeLa SPheres for 7 days for CD49f
Moreover, P-selectin, CD49d, CD49e, CD49f
, and CCR7 all have been identified on the basophil surface and are involved in the IL-3-mediated rolling and adhesion of basophils to endothelial cells .
The single-cell suspension obtained was then incubated with 5% BSA (Sigma) in PBS for 30 minutes at 4C0, washed with 0.1% BSA in PBS, and incubated with an Alexa Fluor 488 1:100 conjugated antibody against the CD49f
surface marker (Biolegend) on ice 1h.
CD105, CD44, CD29, 9 (CD9), CD49f
, CD90, major histocompatibility complex class I (MHC I), multipotent markers octamer-binding transcription factor 4 (Oct-4; the main regulator of differentiation in pluripotent cell line), stage-specific embryonic antigen 4 (SSEA-4; the marker used to differentiate between human and mouse cells and used to characterize pluripotent stem cells), and stromal-derived factor-1 (STRO1; used as identification, isolation, and functional testing of human bone marrow stromal progenitor cells) have been identified under fluorescent microscopy or by flow cytometry.
When A549 cells were stimulated by [H.sub.2][O.sub.2] the levels of the adhesion molecules CD49f
, CD49b, CD29, and CD44 were increased.
The cells consistently differentiated into three lineages in culture, were negative for hematopoietic markers (CD34, CD36, CD117, and CD45), and were positive for mesenchymal markers CD29 (95%), CD44 (>93%), CD49c (99%), CD49f
(>70%), CD59 (>99%), CD90 (>99%), CD105 (>99%), and CD166 (>99%).
Machida and colleagues (2012) also found that TISCs isolated from the livers of alcohol-fed HCV Ns5a transgenic mice and from alcoholic patients carried molecules called CD133 and CD49f
(i.e., were CD[133.sup.+]/CD[49f.sup.+] cells).
Moreover, another report  provided evidence that CD49f
as a stemness marker of BMSCs was also correlated with cell adhesion on laminin-521.
303004; Biolegend), Alexa 488 conjugated-Rat IgG2a against integrin [alpha]6 (CD49f