The characterization of IFP-MSCs is based on the presence of cell markers such as CD9, CD10, CD13, CD29, CD44, CD49e
, CD59, CD105, CD106, and CD166 .
Nine antigens (CD44, CD49e, CD71, CD221, CD227, EGFR, EpCAM, Her2, and Her3) are breast-tumor related and expressed by at least one of the included cell lines (19).
Two outliers were excluded, CD44 and CD49e on HS578T-cells (Fig.
Moreover, P-selectin, CD49d, CD49e
, CD49f, and CCR7 all have been identified on the basophil surface and are involved in the IL-3-mediated rolling and adhesion of basophils to endothelial cells .
FITC-conjugated mouse anti-human CD49e
that reacts with [alpha]5 chain of VLA-5 complex ([alpha]5[beta]1 integrin), FITC-conjugated IgG2 negative control for [alpha]5 subunit from [alpha]5[beta]1 integrin, FITC-conjugated mouse anti-human CD49d that recognizes [alpha]4 subunit of VLA-4 complex ([alpha]4[beta]1 integrin), FITC-conjugated mouse IgG1 negative control for [alpha]4 and [beta]1 subunits, and FITC-conjugated mouse anti-human CD29 antibody that recognizes the [beta]1 subunit of human integrins were purchased from Serotec (UK).
(8,9) Additional positive markers include CD9, CD11c, CD29, CD33, CD43, CD44, CD45, CD49d, CD49e
, CD51, CD54, CD71, and the high-affinity immunoglobulin E receptor.
The expression of CD11b was slightly up-regulated, while no significant changes were observed for other adhesion markers(CD11a,CD18,CD29, CD49d, CD49e
and CD44; data not shown).
Other surface molecules expressed by both ASCs and BM-MSCs include CD13, CD49e
, CD54, CD63, and CD146 [17, 19] (Figure 4).
Flow cytometry analysis demonstrated the dissimilarity between hepatocytes and undifferentiated ADHLSCs as shown by the expression of stromal markers CD90 and CD105, adhesion molecules CD44 and CD49e
, immune regulatory molecules CD73 and HO-1, and NK ligands CD112 and CD155.
, show that MDS cells are heavily dependent on their "dysplastic niche." MDS-derived MSCs display enhanced supportive capacities for clonal hematopoiesis by decreased expression of cell surface molecules , including CD44 and CD49e
In this study, we used flow cytometry to analyse the effects of short-term hypoxic stress on the expression of CD29 (integrin [beta]1), CD44 (HCAM), CD49d (integrin [alpha]4), CD49e
(integrin [alpha]5), CD51 (integrin aV), CD61 (integrin [beta]3), and CD184 (CXCR4).