For the EV Array, the following antibodies were used for capturing: CD11a (HI111) from Ab Biotech (San Diego, CA, USA); Flotillin-1 and TSG101 (5B7) from Abcam (Cambridge, MA, USA); CD3 (HIT3a), CD14 (M5E2), CD16 (3G8), CD28 (L293), CD49d
(L25), and CD56 (3G8) from BD Biosciences (Mountain View, CA, USA); CD41 (HIP8), CD63 (MEM-259), HLA-ABC (W6/32), and Alix (3A9) from Biolegend (San Diego, CA, USA); ICAM-1 (R6.
Al estudiar por citometria de flujo, linfocitos B de pacientes con LLC, tambien se han detectado otros marcadores que han sido relacionados con peor pronostico de la enfermedad: ZAP-70, CD38 (28,29) y CD49d
CLL cells that migrated out through the vessel walls had signicantly higher levels of several molecules on their surface - the protein CD49d
in particular - compared with CLL cells that remained in circulation.
Ademas de los antigenos, para la activacion se usaron los anticuerpos coestimulatorios CD28 (1 [micron]g/ml) y CD49d
(1 [micron]g/ml) (Becton Dickinson [R]).
The expression of CD11b was slightly up-regulated, while no significant changes were observed for other adhesion markers(CD11a,CD18,CD29, CD49d
, CD49e and CD44; data not shown).
New data on ATL/TV1102, a second generation antisense inhibitor of CD49d
, a subunit of VLA-4, for the treatment of RRMS patients, will be presented as well.
8,9) Additional positive markers include CD9, CD11c, CD29, CD33, CD43, CD44, CD45, CD49d
, CD49e, CD51, CD54, CD71, and the high-affinity immunoglobulin E receptor.
In healthy subjects, particle exposure with exercise reduced expression of adhesion molecules CD54 and CD18 on monocytes and CD18 and CD49d
Stimulation was performed with 40 [micro]L/mL (total protein content of approximately 1 [micro]g/mL) of vaccinia viral antigen resuspended according to the manufacturer's instructions (Maine Biotechnology Services, Portland, ME), or 2 [micro]g of CMV antigen (Biowhittaker, Walkersville, MD), always in the presence of co-stimulation with both anti-CD28 and CD49d
monoclonal antibodies (1 [micro]g/mL, Becton, Dickinson and Company, Franklin Lakes, N J).
In addition, clinical data will be presented on the investigational compound ATL/ TV-1102, a second generation antisense inhibitor of CD49d
, a subunit of VLA-4, for the treatment of RRMS patients.
Alternate theories of the mechanism of sequestration of IVLBCL to the intravascular space include deficient expression of receptors for peanut agglutinin and CD49d
, both of which promote homing of tumor cells to endothelial cells via interaction with CD54 and CD106 (CD49d
FITC PE PE-Cy5 APC 1 [kappa] CD34 CD62L CD38 2 [kappa] CD14 CD44 CD38 3 [kappa] CD126 HLA-DR CD38 4 [kappa] CD117 CD128 CD38 5 [kappa] CD11b CD38 CD3 6 [kappa] CD184 CD38 CD19 7 [kappa] CD45ro CD38 CD10 8 [kappa] CD31 CD38 CD54 9 [kappa] CD50 CD38 CD49d
10 [kappa] CD130 CD38 CD44 11 [kappa] CD56 CD38 CD20 12 [lambda] CD34 CD62L CD38 13 [lambda] CD14 CD44 CD38 14 [lambda] CD126 HLA-DR CD38 15 [lambda] CD117 CD128 CD38 16 [lambda] CD11b CD38 CD3 17 [lambda] CD184 CD38 CD19 18 [lambda] CD45ro CD38 CD10 19 [lambda] CD31 CD38 CD54 20 [lambda] CD50 CD38 CD49d
21 [lambda] CD130 CD38 CD44 22 [lambda] CD56 CD38 CD20 Abbreviations: APC, allophycocyanin; FITC, fluorescein isothiocyanate; PE, phycoerythrin; PE-Cy5, phycoerythrin-Cy5.