(5) In addition, a high expression of CD38, 70 kDa zeta-associated protein (ZAP-70), C-X-C chemokine receptor type 4 (CXCR4), and CD49d
is predictive of poor prognosis.
Cell line 1-17 included more neural crest-related marker p75NTR-positive cells (38.41%) than cell line 1-11 (6.26%) (Figure 5(a)) and highly expressed neural crest-related marker genes, p75NTR, SLAG, SOX10, NESTIN, and CD49D
Musial, "Increased expression of alpha(2) (CD49b), alpha (4) (CD49d
) and beta(1) (CD29) integrin subunits on peripheral blood T lymphocytes in clinically stable mild-to-moderate persistent asthma," Polish Archives of Internal Medicine, vol.
Albert et al., "Blockade of CD49d
(alpha4 integrin) on intrapulmonary but not circulating leukocytes inhibits airway inflammation and hyper-responsiveness in a mouse model of asthma," The Journal of Clinical Investigation, vol.
CD13 (BA0718), CD44 (BA0321), CD106 (BA0406), and CD49d
(BA1640) antibodies were from Boster Biological (Wuhan, China).
Al estudiar por citometria de flujo, linfocitos B de pacientes con LLC, tambien se han detectado otros marcadores que han sido relacionados con peor pronostico de la enfermedad: ZAP-70, CD38 (28,29) y CD49d
CLL cells that migrated out through the vessel walls had signicantly higher levels of several molecules on their surface - the protein CD49d
in particular - compared with CLL cells that remained in circulation.
Ademas de los antigenos, para la activacion se usaron los anticuerpos coestimulatorios CD28 (1 [micron]g/ml) y CD49d
(1 [micron]g/ml) (Becton Dickinson [R]).
Typically, aberrant antigen profiles in neoplastic plasma cells include expression of CD28, CD56, CD20, CD117 (less commonly CD13, CD33, CD44, or CD49d
), diminished CD27, diminished CD38, and the complete absence of CD19 and/or CD45.
FITC-conjugated mouse anti-human CD49e that reacts with [alpha]5 chain of VLA-5 complex ([alpha]5[beta]1 integrin), FITC-conjugated IgG2 negative control for [alpha]5 subunit from [alpha]5[beta]1 integrin, FITC-conjugated mouse anti-human CD49d
that recognizes [alpha]4 subunit of VLA-4 complex ([alpha]4[beta]1 integrin), FITC-conjugated mouse IgG1 negative control for [alpha]4 and [beta]1 subunits, and FITC-conjugated mouse anti-human CD29 antibody that recognizes the [beta]1 subunit of human integrins were purchased from Serotec (UK).
The expression of CD11b was slightly up-regulated, while no significant changes were observed for other adhesion markers(CD11a,CD18,CD29, CD49d
, CD49e and CD44; data not shown).
This important member of the [beta]1 integrin family is composed of two chains: CD49d
(alpha 4) encoded by ITGA4 gene and CD29 (beta 1) encoded by ITGB1.