Moravek et al., "Human uterine leiomyoma stem/progenitor cells expressing CD34 and CD49b
initiate tumors in vivo," The Journal of Clinical Endocrinology & Metabolism, vol.
Musial, "Increased expression of alpha(2) (CD49b
), alpha (4) (CD49d) and beta(1) (CD29) integrin subunits on peripheral blood T lymphocytes in clinically stable mild-to-moderate persistent asthma," Polish Archives of Internal Medicine, vol.
MSCs were characterized by standard markers using fluorescence-activated cell sorting (FACS): MHC molecules (HLA-ABC+) and adhesion molecules (CD44+, CD54 (low), CD90+, CD106+, CD29+, CD49b
(low), and CD105); however, they were negative for hematopoietic markers (CD34-, CD45-, and HLA-DR-) and the marker CD117 (Dominici et al.
NK cells were separated from spleens using Mouse panNK CD49b
Selection Kit (Cat.
The cells (5 x [10.sup.5] cells/25 [micro]l) from the splenocytes were stained with 10 [micro]l of FITC-labelled anti-mouse CD4, PE-labelled CD8, PE-Cy5-labelled anti-mouse CD3, PE-Cy5 anti-mouse CD3e, FITC-labelled anti-mouse CD49b
, PE-labelled NK-T, FITC-labelled anti-mouse CD14, PE-labelled CD11 c, PE-Cy5-labelled CD137, FITC-labelled CD86, PE-Cy5-labelled MHC11 at 4[degrees]C for 30 min in the dark.
Briefly, non CD4-expressing cells are labeled with a cocktail of biotin-conjugated antibodies against CD8a, CD45R, CD11b, CD25, CD49b
, TCR[gamma]/[delta], and Ter-119 and antibiotin microbeads.
For example, several integrins such as [[alpha].sub.1] integrin (CD49A), [[alpha].sub.2] integrin (CD49B
), [[alpha].sub.2b] integrin (CD41), and [[beta].sub.1] integrin (CD29) were upregulated in the liver metastasizing cell line, whereas angiopoetin 1 and MMP10 were upregulated genes of the peritoneal disseminating cell line.
These include three main kinds of T cells: Tr1 cells, a population activated in the periphery after antigenic stimulation in the presence of IL-10 and which express the surface markers LAG-3 (lymphocyte-activation gene 3) and CD49b
in the face of absent FOXP3 and CD25 expression; Th3 cells, which are also differentiated in the periphery and these Tregs mediate the cell suppression by secreting the cytokine TGF-[beta]; and finally, [CD8.sup.+] Tregs , described as antigen-specific memory T cells with Treg properties, which may regulate immune responsiveness by production of IL-10, TGF-[beta]1, and IFN[gamma] though the exact mechanisms underlying this suppression are still largely unknown.
ADSCs do not have a specific marker and the expression of antigens is similar to bone marrow MSCs: CD10, CD13, CD29, CD34, CD44, CD54, CD71, CD49b
, CD90, CD105, CD117, and STRO-1.
When A549 cells were stimulated by [H.sub.2][O.sub.2] the levels of the adhesion molecules CD49f, CD49b
, CD29, and CD44 were increased.
Another frequently used marker for these cells in NK1.1- mice strains is the antibody DX5, which recognizes the [[alpha].sub.2]-integrin CD49b