According to the manufacturer's instructions, the non-T-helper cells as well as regulatory T cells and [gamma]/[delta]T cells were depleted by indirect magnetic labeling using a cocktail of lineage-specific biotin-conjugated antibodies against CD8a, CD45R, CD49b
, CD11b, and Ter-119, as well as antibodies against CD25 and TCR[gamma]/[delta] in combination with Anti-Biotin MicroBeads.
2] the levels of the adhesion molecules CD49f, CD49b
, CD29, and CD44 were increased.
5] cells/25 [micro]l) from the splenocytes were stained with 10 [micro]l of FITC-labelled anti-mouse CD4, PE-labelled CD8, PE-Cy5-labelled anti-mouse CD3, PE-Cy5 anti-mouse CD3e, FITC-labelled anti-mouse CD49b
, PE-labelled NK-T, FITC-labelled anti-mouse CD14, PE-labelled CD11 c, PE-Cy5-labelled CD137, FITC-labelled CD86, PE-Cy5-labelled MHC11 at 4[degrees]C for 30 min in the dark.