Primary antibodies were as follows: rabbit polyclonal antibody to CD45
(Abcam, Cambridge, Massachusetts), rabbit monoclonal antibody to hemoglobin [alpha] (Boster, Wuhan, China), mouse monoclonal antibody to CD68 (Abcam), and mouse monoclonal antibody to PM-2K (Abcam).
Single Doses of Antibody Drug Conjugates Targeted to CD117 or CD45
Have Potent In Vivo Anti-Leukemia Activity and Survival Benefit in Patient-Derived AML Models.
The vascular endothelial cells were identified by CD31 (mouse, Zsbio, ZM-0044, 1:100); the hematopoietic cells (lymphocytes and leukocytes, etc.) were identified by CD45
(mouse, Zsbio, ZM-0183, 1:100); the lymphatic vessels were identified by D2-40 (mouse, DAKO, IS07230-2, ready to use); and the nervous tissues were identified by S-100 (mouse, Servicebio, GB13361, 1:200).
A mixture of padlock probes targeting the housekeeping gene ACTB, KRAS codon 12wt, andmutG12S, G12R, G12C, G12D, G12V, and G12A were combined with immunofluorescence staining of cytokeratin and CD45
The following primary antibodies were used: Brilliant Violet 421[TM] Rat AntiMouse CD44 (BD Biosciences), Fluorescein Isothiocyanate (FITC) Rat Anti-Mouse CD90.2 (BD Biosciences), PerCP/ Cy5.5 Anti-Mouse CD34 (BioLegend, San Diego, CA, USA), and PE/Cy7 Rat Anti-Mouse CD45
The established criteria for identification of CTCs such as DAPI positivity, cytokeratin positivity, and CD45
negativity  demonstrate only that the isolated cells show traces of being epithelial and not of being an immune cell, but do not in and of themselves demonstrate that the cell is a cancer cell.
Eventually the patient underwent left pneumonectomy with final histology including immunohistochemistry demonstrating anaplastic large cell lymphoma positive for CD30, Ki-67, CD45
, and ALK-1 (Figure 4).
Serum-free protein block (Dako Denmark A/S, Glostrup, Denmark) was used to decrease nonspecific conjugate binding, and primary antibodies (rabbit polyclonal to PAI-1 (ab66705) 1: 200, mouse monoclonal to CD45
(ab30470) 1:100, mouse monoclonal to CD68 (ab955) 1: 100, and mouse monoclonal to alpha-smooth muscle actin (a-SMA; ab7817) 1:100; Abcam, Cambridge, UK) were incubated at 4[degrees]C overnight.
In the left panel: dot plots depicting SSC-A X CD45
expression (panleukocyte marker) of tumors from WT and PAFR KO animals.
Hence, scarce data are available on the immunohistomorphology of microvascular ECs for CD34 and the expression of CEPCs for commonly used EC markers like CD31, CD34, CD45
and CD133 in the pancreas tissue in a diabetic rat, in comparison with that of the kidneys and liver.
The results were as follows: Flow cytometric immunophenotyping identifies a phenotypically distinct population of cells with low light scatter properties that express CD19, low density CD20, CD5, and CD45
and display Kappa immunoglobulin light chain restriction.