Hence, scarce data are available on the immunohistomorphology of microvascular ECs for CD34 and the expression of CEPCs for commonly used EC markers like CD31, CD34, CD45
and CD133 in the pancreas tissue in a diabetic rat, in comparison with that of the kidneys and liver.
The results were as follows: Flow cytometric immunophenotyping identifies a phenotypically distinct population of cells with low light scatter properties that express CD19, low density CD20, CD5, and CD45
and display Kappa immunoglobulin light chain restriction.
The expressions of CD90 for mesenchymal and CD34 and CD45
for hematopoietic markers were evaluated via RT-PCR.
The cells that were positive for vimentin/cytokeratin and estrogen/progesterone receptor and negative for CD45
were identified as CECs.
Among the 10 cases of the cytological diagnosis of lymphoma, 7 cases were reported as non-Hodgkin's lymphoma and 3 cases were reported as Hodgkin's lymphoma in the histopathological examination and also showed positivity for CD45
, CD19 and CD20 by NHL and CD15, CD30 positivity by HL in IHC study.
69) According to the authors, (57, 69) expression of CD20, CD45
, and CD56 was detected in 9.
Third-passage DFSCs were incubated with the antibodies of positive markers for MSCs of human CD29 allophycocyanin (APC), CD73 phycoerythrin (PE), CD105 PE, and CD146 fluorescein isothiocyanate (FITC) and with the antibodies of negative markers of CD14 PE, CD34 FITC, CD45
APC, and HLA-DR PE (BD Biosciences, USA) for 15 min at room temperature in the dark.
The cell suspensions are further labeled with antibodies to CD45
, CD8, CD3, PD-L1 and then stained with a Cell Cycle Dye.
BC8 has been developed to target CD45
, a pan-leukocytic antigen widely expressed on white blood cells.
After depletion of red blood cells (RBCs), cells from vehicle-exposed CD45
BMSCs expressed CD90 marker of mesenchymal stem cells but not CD34 and CD45
as markers for hematopoietic stem cells (Fig.
5] cells were suspended in PBS and were incubated with anti-rat monoclonal antibodies for CD29, CD45
, CD90, and CD105.