Mechanism and biological significance of CD44
The samples were blocked by 10% (v/v) goat serum for 30 min, then incubated in 1% BSA containing the following polyclonal antibodies: (1) rabbit anti-sheep Oct-4 (1:100); (2) rabbit anti-sheep CD29 (1:100); (3) rabbit anti-sheep CD44
(1:100); (4) rabbit anti-sheep CD71 (1:100); (5) rabbit anti-sheep CD73 (1:100) and (6) rabbit anti-sheep CD90 (1:100) for 1h at room temperature.
The samples were analyzed by immunohistochemistry of CD29, CD44
, CD90, and CD45 and observed by light microscopy. Fibroblasts were also cultured on glass coverslips in six-well plates for 7 days and then analyzed by immunohistochemistry of fibroblast-specific protein-1 (FSP-1).
Hyaluronan-mediated angiogenesis in vascular disease: uncovering RHAMM and CD44
receptor signaling pathways.
Flat intraurothelial neoplasia exhibiting diffuse immunoreactivity for CD44
and cytokeratin 5 (urothelial stem cell/basal cell markers): a variant of intraurothelial neoplasia commonly associated with muscle-invasive urothelial carcinoma [published online ahead of print March 17, 2017].
Flow cytometry was performed using markers of mouse MSCs (CD44
, CD90.2), hematopoietic stem cells (CD34), and leukocytes (CD45).
Consistent with CD13 expression, the combination of IL-1[beta] + TNF-[alpha] (p [less than or equal to] 0.01) and IL-6 + IL-1[beta] + TNF-[alpha] (p [less than or equal to] 0.05) [+ or -] TIMP-1 (p [less than or equal to] 0.01) induced a significantly higher CD44
expression in CB-derived [CD34.sup.+] cells (>2-fold increase, respectively; Figure 1(d)).
Yeh et al., "Differential expression of CD44
and CD24 markers discriminates the epitheliod from the fibroblastoid subset in a sarcomatoid renal carcinoma cell line: evidence suggesting the existence of cancer stem cells in both subsets as studied with sorted cells," Onco-target, vol.
Hyaluronic acid (HA) binds to its receptor (CD44
) to participate in the regulation of tumor growth and metastasis.
Additionally, fluorescent reporter assay was used to clear the targeting site of micro ribonucleic acid 34-a and CD44
. Furthermore, protein levels of CD44
, the putative target gene of micro ribonucleic acid 34-a, was assessed after transfection by Western blot.
One accepted marker of cancer stem cells is CD44
, the receptor for hyaluronic acid (HA).
SNP correlated with TNF) [21-23] and presence of H blood group antigens on CD44