They may be negative for CD5, CD10 and CD23 and positive or negative for CD43
Various Gal 1 ligands are found on lymphocytes (CD7, CD43
, and CD45), on endothelial cells (CD13, CD36, ROBO4, and integrins), and in the extracellular matrix (fbronectin, integrins, laminin, ROBO4, and GM1) (14).
Based on immunohistochemistry, the cells were discovered to express leukocyte common antigen (LCA), CD4, CD43
, CD56, terminal deoxynucleotidyl transferase (TdT), S100, and CD123.
(37) Primary cutaneous marginal zone lymphomas lack expression of BCL6, CD10, and CD5 but may express CD43
, unlike PCFCL.
After transient overexpression of hematopoietic transcription factors (TFs), we generated more [CD34.sup.+] cells with normal expression level of CD43
and CD31, which were used to define hematopoietic progenitors in hPSCs/OP9 coculture system (15).
Tdt, CD5, CD3, and CD43
were positive, while CD34, CD117, Pax5, Bcl2, CD20, and CD23 were negative (Figure 4).
Tong et al., "ERM-dependent movement of CD43
defines a novel protein complex distal to the immunological synapse," Immunity, vol.
We evaluated the immunostaining for NF-[kappa]B (p65) using ImageJ software, and the results were expressed as percentages, while the quantification for ED-1 and CD43
was performed by counting positive cells in 30 fields (0.245 [mm.sup.2]) of the tubulointerstitial compartment of each histological slide.
The flow cytometry analysis detected an abnormal T-cell phenotype expressing CD45, cCD3, bright surface CD7, CD43
, partial CD2, two myeloid markers (CD13 and CD33), dim/partial TdT, and, to a lesser extent, CD1a, while lacking sCD3, CD4, CD8, CD5, and defining markers for myeloid, monocytic, and B-lymphoid lineage (MPO-, CD14-, CD64-, CD11b-, CD19-, and CD20-) (Figure 2(c)).
In immunohistochemical studies, Vimentin, S-100, CD1a, HMB 45, CD34, CD43
, CD13, CD23, CD21, CD117, and MPO were detected to be negative.
B-cell markers such as CD10, CD20, CD43
, CD45, CD79a, CD99, BCL2, BCL6, MUM-1, and terminal deoxynucleotidyl transferase are helpful in establishing a diagnosis [3, 20].
Immunohistochemical stains were positive for CD20 (++) (Figure 4(b)), CD3 (+), CD5 (+), CD43
(+), CD10 (+), Ki67 (10%) anti BCL-6 (+), MUM-1 (+), and CD138 (+), whereas they were negative for CD1.