The primary panel of monoclonal antibodies (mAbs) used were CD45 (for blast identification), CD13, CD33, CD34, HLA-DR and for AML M4/M5 CD14, for AML M6 anti-glycophorin A and for AML-M7, CD61, CD41 and CD42
In addition, targeted phenotyping was limited to detection of the surface marker for CD62E and CD42
. CD62E is found on activated endothelium, but other endothelial markers might reflect better the endothelial changes during exercise.
El hibrido CD42
presento la mayor altura de planta (99,58cm) y fue estadisticamente similar a CD50 (83,71cm), vigor hibrido no deseado en un genotipo de buenas caracteristicas para nochebuena; sin embargo, comparado con los hibridos restantes se comporto diferente, El CD34 mostro el menor porte con 68,65cm, Los demas materiales presentaron valores estadisticamente iguales,
EPCs phenotype was confirmed by the expression of the main endothelial cell surface markers such as CD14 (98.90 [+ or -] 0.46%), CD31 (41.81 [+ or -] 23.17%), CD34 (40.20 [+ or -] 32.62%), CD42
(1.18 [+ or -] 1.62%), CD45 (96.61 [+ or -] 3.71%), CD133 (8.65 [+ or -] 6.38%), and VEGFR-2 (43.18 [+ or -] 20.35%).
EMPs were characterized by size <1[micro]m and bound to CD31 but not to CD42
Phenotypically, blasts commonly express stem cell (variable CD34, CD117), myeloid (CD13, CD33), nonlineage (CD4, CD7, CD56), and megakaryoblastic/megakaryocytic (CD61, CD41, CD42
The PPP was incubated for 20 min with fluorescently labeled mouse anti-human CD42
FITC and CD31 PE (BD Biosciences) antibodies for PMP identification, and with CD51 FITC (BD Biosciences) to detect EMP.
The peripheral blood and bone marrow blasts in TAM have basophilic cytoplasm with cytoplasmic blebbing, suggestive of megakaryoblasts.2 Blasts in TAM display a characteristic immunophenotype.9 In most cases the blasts are positive for CD34, CD56, CD117, CD13, CD33, CD7, CD4 (dim), CD4, CD42
, TPO-R, CD36, CD61 and CD71.The blasts are negative for MPO, CD15, CD14 and glycophorin A.
We incubated 10 [micro]L EMP suspension with 10 [micro]L antihuman/rat E-selectin, CD144, CD31, and CD42
or isotype control.
Examples might include assessment of in vivo platelet activation in whole blood by the identification of platelet activation markers such as p-selectin (CD62-p), which is not found on the surface of the resting platelet, and measurement of the decrease in the platelet surface expression of glycoprotein lb-V-IX complex (CD42
) on platelet activation.
However, the megakaryocytes and megakaryoblasts highlighted by CD42
immunohistochemical stain (1:100; clone MM2/174, Novocastra, Buffalo Grove, Illinois; Figure 2), comprised approximately 60% of marrow cellularity.
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