EPCAM

(redirected from CD326)

EPCAM

A gene on chromosome 2p21 that encodes a carcinoma-associated antigen expressed on most normal epithelial cells and gastrointestinal carcinomas, which functions as a homotypic calcium-independent cell adhesion molecule. EPCAM is thought to function as a relay molecule between intestinal epithelial cells and intraepithelial lymphocytes at the mucosa, providing an immune barrier to infection. It is of interest in oncology as a target for cancer immunotherapy. It upregulates expression of FABP5, MYC and cyclins A and E.

Molecular pathology
Defects in EPCAM cause congenital tufting enteropathy (diarrhoea type 5).
References in periodicals archive ?
Gires, "EpCAM (CD326) finding its role in cancer," British Journal of Cancer, vol.
Mellstedt, "Epithelial cell adhesion molecule expression (CD326) in cancer: A short review," Cancer Treatment Reviews, vol.
EpCAM (CD326), a 40kDa transmembrane glycoprotein, is overexpressed in many solid tumors, while it shows variable but generally low levels in the normal epithelium.
Table 1: Details of PCR and RFLP procedures and expected products for hOGGI and APE1 genes Gene Primers PCR conditions PCR Forward/Reverse Product hOGGI 5'-ctg ttc agt 95[degrees]C- 5 m, 247 bp (C1245G) gcc gac ctg cgc 35 cycles of cga-3' 95[degrees]C-30 s, 64[degrees]C-30 s, Ser326Cys 5'-atc ttg ttg 72[degrees]C-30 s, cd326 tgc aaa ctg 72[degrees]C-5 m Exon7 ac-3' APE1 5'-ctg ttt cat ttc 95[degrees]C-5 m, 164 bp tat agg cta-3' 35 cycles of (T2197G) 5'-agg aac ttg 95[degrees]C-20 s, cga aag gct 55[degrees]C-20 s, Asp148Glu tc-3' 72[degrees]C-20 s, cd 148 72[degrees]C-5 m Exon5 Gene Restriction Restriction products enzyme hOGGI 2U of MbolI Ser/Ser: 224bp, 23bp (C1245G) Ser/Cys: 247bp, Ser326Cys 224bp, 23bp cd326 Cys/Cys: 247bp Exon7 APE1 1U of Bfal Asp/Asp: 144 bp.
Expression of surface molecules such as CD29, CD44, CD73, CD90, CD105, CD166, and human leukocyte antigen- (HLA-) ABC was positive in both donor- and RR-MS patient-derived hPDLSCs at P2 and P15 passages (Figure 1(a)), while that of CD14, CD31, CD34, CD45, CD326, and HLA-DR was negative (data not shown).
In order to find putative impurities in the cell culture, cells were incubated in the dark for 30 minutes with fluorescein isothiocyanate- (FITC-) conjugated monoclonal antibodies against CD31(PECAM-1) (number F8402, Sigma-Aldrich), a marker of endothelial cells, and CD326 (EpCAM) (number 342203, BioLegend), a marker of epithelial cells.
confirmed that spheres from clinical specimens showed self-renewal, clonogenic, and tumorigenic potential and identified that they had lower expression levels of CD326, CD24, CD44, and higher ABCG2 [49].