Labeled and unlabeled T/cell populations are compared for the presence of the following phenotypic markers: CD3, CD4, CD8, and CD32
. (a) Histograms comparing cellular populations labeled with conjugated antibodies for the depicted surface marker or isotype control.
In the subtitle of the print product, Gale itemizes a long list of optical disc formats covered, and indeed there are a few hundred records for CD-I, 3D0, MMCD, and CD32
Luster, "Human lupus autoantibody-DNA complexes activate DCs through cooperation of CD32
and TLR9," The Journal ofClinical Investigation, vol.
Also, because CRP appears to mediate its effects in HAECs via CD32
and CD64, we pretreated HAECs with blocking antibodies to CD16, CD32
, and CD64 before treatment with CRP.
Regulatory roles for FcgammaRIII (CD16) and FcgammaRII (CD32
) in the development of T- and B-lineage lymphoid cells.
Briefly, [10.sup.6] splenocytes or 100 [micro]L of whole blood was pretreated for 10min with FC-Block [100 [micro]L in staining buffer containing 0.5 [micro]g of anti-rat CD32
(BD Biosciences, San Jose, CA)] to prevent nonspecific Fc receptor-mediated antibody binding.
Fc[gamma] receptors CD16, CD32
, and CD64 have been reported as the receptors for CRP (7-9).
Ravetch, "Structure and expression of human IgG FcRII(CD32
) functional heterogeneity is encoded by the alternatively spliced products of multiple genes," The Journal of Experimental Medicine, vol.
Nine candidates failed the initial criteria, such as assay length or unsuitable CpG site location for primer and probe placement [BCOR, BCL6 co-repressor; CAV1, caveolin 1, caveolae protein, 22kDa; CD44, CD44 molecule (Indian blood group); CDH13, cadherin 13, H-cadherin (heart); VCAN (formerly CSPG2), versican; FCGR2A, Fc fragment of IgG, low affinity IIa, receptor (CD32
); GSK3B, glycogen synthase kinase 3 beta; PCDH17, protocadherin 17; and TAF11, TAF11 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 28kDa].
Specifically, the inhibition of blastogenesis and proliferation of activated T and B cells by IVIg is attributed to Fc receptors for IgG (Fc[gamma]RI (CD23), FcyRII (CD32
), Fc[gamma]RIII (CD16), and Fc[epsilon]R1 (CD64)) expressed on the immune cells upon activation .
In that study, 55 children with recurrent infections were evaluated for several factors of the immune system: IgG, IgA, IgM, and IgG subclass concentrations; MBL2 genotype; IgG2 subclass allotype (GM); partial C4 and C2 deficiency; FCGR2A [Fc fragment of IgG, low affinity IIa, receptor (CD32
)] polymorphism; and the specific antibody response to the pneumococcal vaccine Pneumovax.
The frequencies of CD11b, CD11c, and CD32
were also analyzed by combination with CD206, CD209, and Dectin-1.