The number of microvessels indicated by the endothelial marker CD31
staining in miR-199a-overexpressing tumors was significantly lower than in the control (Figure 2C).
Immunohistochemically, tumor cells were strongly reactive for vimentin and pancytokeratin (AE 1-3), but negative for desmin, myoglobin, S100, melanoma cocktail, TTF-1, CK7, CK20, CDX2, PAX8, CD31
, CD34, Factor VIII, CD30, and CD45.
The spindle cells showed positive reaction with CD34, CD31
, Factor VIII and HHV-8 (Figs.
Expression of Ki-67 and CD31
was analysed in formalin fixed, paraffin-embedded sections with the method of indirect immunoperoxidase with the use of polyclonal rabbit antibodies to human antigen Ki-67 and CD31
Previously, using this model, endothelial cell markers Von Willebrand factor (vWF) and CD31
were localised to the neovessels, confirming that the outgrowths were indeed blood vessels.
Immunohistochemical analyses of angiogenic relate antigens: PECAM or CD31
and CD68 in MT were carried out.
cells lacking markers CD2, DC3, CD10, CD16, CD18, CD31
, CD64 and CD140b), isolated from one primary breast cancer and eight malignant pleural effusion samples, were able to form heterogeneous tumours containing both the CD[44.
Some of the spindle cells were considered positively stained with CD31
, factor VIII and alpha-1-antitrypsin.
The surgical resection showed a multilocular cyst having a flattened cell lining that was immunopositive for CD31
The most useful stain is CD31
, which confirms the endothelial derivation of the neoplastic cells, he added.
Endothelial cells and type A synoviocytes expressed CD31
and CD36 in OA, but only endothelium in normal subjects.
specifically targets CD31
, a target that has so far proved intractable