Marker Fluorochrome Host Clone CD14 PE Mouse anti-human M[phi]P9 CD16 FITC Mouse anti-human B73.1 CD34 FITC Mouse anti-human 581 CD45 PE Mouse anti-human HI30 CD133 APC Mouse anti-human AC133 CD235a FITC Mouse anti-human GA-R2 (HIR2) CD309
PE Mouse anti-human 89,106 Marker Manufacturer CD14 Becton Dickinson Bioscience CD16 Becton Dickinson Bioscience CD34 Becton Dickinson Bioscience CD45 Becton Dickinson Bioscience CD133 Miltenyi Biotec CD235a Becton Dickinson Bioscience CD309
Becton Dickinson Bioscience PE: phycoerythrin; FITC: fluorescein isothiocyanate; APC: allophycocyanin.
Cell origin of microvesicles was determined using lineage specific antigen labeling with CD105 AF647 (BD Biosciences), platelet and endothelial cell adhesion molecule 1 (PECAM1)/CD31 BV510 (BD Biosciences), VEGFR2/ CD309
AF647 (BD Biosciences), and mucosal vascular addressin cell adhesion molecule 1/MadCAM APC (Biotechne R&D Systems).
The next most manifest response was the level of expression of CD309 (VEGFR-2), which is known to increase endothelial permeability in microvascular bed .
An increase in CD309 expression leads to increase of endothelial permeability in microvascular bed .
Mononuclear cells (5 x [10.sup.6]) were labeled with preconjugated mouse anti-human monoclonal antibodies: CD34 conjugated to allophycocyanin, CD309 (VEGF receptor-2 [VEGFR2]/kinase insert domain-containing receptor [KDR]) to fluorescein isothiocyanate (BD Biosciences, USA), and CD45 to peridinin chlorophyll protein-cyanine for 20 min at room temperature.
C, Negative control, and D, cells co-expressing CD34 and CD309 were designated as cEPCs.
For analysis of endothelial progenitor cells (EPCs), fresh whole blood EDTA-anticoagulated samples (100 [micro]L) were incubated for 30 minutes at room temperature with the following monoclonal antibodies: 20 [micro]L of FITC anti-human CD34, 5 [micro]L of PE anti-human CD144 (VE-cadherin), or 5 [micro]L of PE antihuman CD309
(VEGFR-2) (BD PharMingen, Erembodegen, Belgium).
MSCs do not express CD31, CD144, and CD309
(endothelial cell markers) or CD14, CD34, CD45, CD117, and CD133 (hematopoietic cell markers) [61, 79].
The human KDR gene, for example, has been known by many different symbols (e.g., VEGFR2, CD309
, FLK1, VEGFR).
BFP-DFAT cells have been shown to be positive for CD90, CD105 , CD13, CD29, and CD44  but negative for CD11b (monocyte marker), CD34 (hematopoietic progenitor cell marker), CD45 (leukocyte common antigen) , CD31, CD309
, CD106, and alpha-smooth muscle actin [15, 30].