For comparison, we tested the expression of surface markers CD3
, CD8, CD16, and CD56 after incubation with each inducer (Figures 1(b) and 1(c)).
[ALK.sup.-] ALCL.--Of the 8 cases analyzed, 6 (75%) expressed at least one T-cell antigen, and in 2 cases, findings were negative for T-cell antigens (1 case assessed only with CD3
To achieve this, the DART molecule combines a portion of an antibody recognizing CD3
, an activating molecule expressed by T cells, with an arm that recognizes CD123 on the target cancer cells.
These results indicate that R-CIK cells activated by RetroNectin have much stronger proliferative ability than only CD3
activated CIK cells.
Immunohistochemically, the neoplastic cells showed CD3
cytoplasmic positivity on paraffin sections, whereas monoclonal CD3
was negative on frozen sections.
Aptevo Therapeutics announced the presentation of new data for APVO436, a bispecific antibody candidate targeting CD123 and CD3
, at the American Association for Cancer Research 2018 Annual Meeting.
According to MacroGenics, MGD006 is a humanized Dual-Affinity Re-Targeting (DART) molecule in development for hematologic malignancies that recognizes both CD123 and CD3
. In pre-clinical studies, MGD006 was shown to redirect T cells via their CD3
component to kill CD123-expressing leukemia cells at very low concentrations of the DART.
Antigen retrieval by microwave procedure in citric acid buffer (10 mM, pH 6.0) for 10 minutes preceded staining for CD3
, CD8, CD20, CD30, CD43, CD45RB, CD56, CD68, CD79A, CD99, Ki67, TIA-I, and TdT.
In another study, the anergy of T lymphocytes induced by a low molecular weight material present in a human placental supernatant was linked to the defective phosphorylation of TCR CD3
A panel of monoclonal antibodies was used specific for the following antigens: CD2, CD3
, CD4, CD7, CD8, CD10, CD13, CD19, CD33, CD34, CD38, CD45 (LC, A), CD56, CD64, CD117 (c-kit), HLA-DR, and terminal deoxynucleotidyl transferase (TdT).