The presence of CD29
, CD44, and CD90 and the absence of hematopoietic and endothelial markers, CD34 and CD45, were used for ADSC identification.
The cells presented high positivity for the following expressions: CD29
Third-passage DFSCs were incubated with the antibodies of positive markers for MSCs of human CD29
allophycocyanin (APC), CD73 phycoerythrin (PE), CD105 PE, and CD146 fluorescein isothiocyanate (FITC) and with the antibodies of negative markers of CD14 PE, CD34 FITC, CD45 APC, and HLA-DR PE (BD Biosciences, USA) for 15 min at room temperature in the dark.
The minimum criteria to define MSCs are your ability to adhere to plastic when kept under cultivation conditions, and present surface antigen expression patterns including CD29
, CD44 (WAGNER et al.
Third, a number of gene targets identified by experts during the 2011 NTP workshop as relevant to the biological processes described in this article are not included in ToxCast[TM], including glucose transporter 2 (GLUT2), insulin receptor substrates 1 and 2 (IRS1, IRS2), the ZFP423 gene and Wnt genes involved in adipogenesis, leptin receptor (LEPR), fatty acid binding protein 4 (FABP4, found in adipocytes), and genes expressed in stem cells that populate white adipose tissue lineage and could be early indicators of commitment to adipocyte lineage (CD24, CD29
, CD34, PDGFRb, NG2, Sca1).
5] cells were suspended in PBS and were incubated with anti-rat monoclonal antibodies for CD29
, CD45, CD90, and CD105.
CD105, CD44, CD29
, 9 (CD9), CD49f, CD90, major histocompatibility complex class I (MHC I), multipotent markers octamer-binding transcription factor 4 (Oct-4; the main regulator of differentiation in pluripotent cell line), stage-specific embryonic antigen 4 (SSEA-4; the marker used to differentiate between human and mouse cells and used to characterize pluripotent stem cells), and stromal-derived factor-1 (STRO1; used as identification, isolation, and functional testing of human bone marrow stromal progenitor cells) have been identified under fluorescent microscopy or by flow cytometry.
Together with CD29
, CD49d forms the very late antigen-4 (VLA-4) that is involved in leukocyte trafficking, adhesion, and extravasation [46, 47].
2] the levels of the adhesion molecules CD49f, CD49b, CD29
, and CD44 were increased.
The phenotype of the MSCs was confirmed by positive expression of Sca-1 and CD29
, and negative for CD45.
To address these shortcomings, the researchers obtained surgical biopsies of muscles of the head, trunk and leg, and used antibody staining to show that human satellite cells can be identified by their co-expression of the transcription factor PAX7 with surface proteins CD56 and CD29
In addition, MSCs were identified by surface marker CD45 (-ve), CD90 (+ve) and CD29
(+ve) detected by flow cytometry (Figure 4A,B and C) respectively.