FT596 is derived from a clonal master induced pluripotent stem cell line engineered with three functional modalities designed to optimize anti-tumor activity: a proprietary CAR targeting B-cell antigen CD19
; a novel high-affinity 158V, non-cleavable CD16 Fc receptor; and an interleukin-15 receptor fusion.
ADCT-402 (loncastuximab tesirine) is an antibody drug conjugate comprised of a humanized monoclonal antibody that binds to human CD19
, conjugated through a linker to a pyrrolobenzodiazepine dimer toxin.
Immunotherapy company NantKwest Inc (Nasdaq:NK) said on Monday that it plans to launch its first-in-human clinical trial targeting CD19
t-haNK in advanced B-cell lymphoma following the US FDA review of its investigational new drug application (IND).
Global Banking News-June 4, 2019-NantKwest to begin first-in-human clinical trial targeting CD19
t-haNK in advanced B-cell lymphoma
the CAR), consisting of an extracellular antigen-recognising receptor (targeting CD19
on B-cells in this therapeutic application) coupled to intracellular signalling domains that allow T-cell activation following recognition of the antigen.
B-cell malignancies express specific tumour associated antigens including CD19
. T-cells can be genetically engineered to express a chimeric antigen receptor (CAR) that recognizes the B-cell antigen CD19
is expressed on developing and mature B-cells, absent on pluripotent haematopoietic stem cells and highly retained over the process of neoplastic transformation.
negative precursor B acute lymphoblastic leukemia (B-ALL)-Immunophenotypic challenges in diagnosis and monitoring: A study of three cases.
After PBMC isolation, the resulting cell suspension was distributed into individual tubes with an amount of 1 x [10.sup.6]/sample, and the samples were incubated with a combination of the following mAbs: CD45 FITC/CD14 PE, Cy-Chrome Mouse Anti-Human CD3, FITC Mouse Anti-Human CD19
, FITC Mouse Anti-Human CD4, FITC Mouse Anti-Human CD8, PE Mouse Anti-Human CD279 (PD-1), and PE Mouse Anti-Human CD274 (PD-L1), all of which came from BD Biosciences (USA).
Single-cell suspensions of murine splenocytes were prepared by manual disruption of total spleens, and highly purified B lymphocytes were isolated by immunomagnetic positive selection according to the manufacturer's instructions (STEMCELL Technologies, EasySep[TM] Mouse CD19
positive selection kit II, Grenoble, France).
The results were as follows: Flow cytometric immunophenotyping identifies a phenotypically distinct population of cells with low light scatter properties that express CD19
, low density CD20, CD5, and CD45 and display Kappa immunoglobulin light chain restriction.
The peripheral blood mononuclear cells were isolated by standard Ficoll-Paque Plus density-gradient centrifugation, and the following monoclonal antibodies were used for human fluorescence-activated cell staining according to the manufacturer's instruction: APC-Cy7-conjugated antihuman CD19
(HIB19), PE-Cy7-conjugated anti-human CD5 (LT21), APC-conjugated anti-human CD1d (51.1), and PerCP-Cy5.5-conjugated anti-human CD23 (EBVCS-5), all from Biolegend.