[ELR.sup.-] chemokines are commonly angiostatic, but CXCL12 (previously known as stromal cell-derived factor 1, abbreviated as SDF1)  and its receptor, CXCR4 (previously known as LESTR, fusion, or CD184
), are reported to promote angiogenesis and play a major role in metastasis.
The immunophenotypic profile was analyzed using the antibodies CD14, CD34, CD44, CD45, CD73, CD90, CD146, CD184, Stro-1 and HLA-DR (Becton Dickinson, USA) conjugated with FITC or PE fluorochrome.
Some antigens presented low expression: CD34 (3.15 [+ or -] 1.75%), CD184 (0.6 [+ or -] 0.6%), and STRO-1 (2.8 [+ or -] 2.5%) and others presented negative expression: CD14, CD45 and HLA-DR.
Chemokine receptors CXCR4 (CD184
) and CCR5 (CD195) are predominant coreceptors for HIV-1 entry into human's cells.
Schwartz et al (20) demonstrated high levels of the neural stem cell markers like CD133, CD44, CD81, CD184
, CD90, and CD29 positive but CD133 cell population was decreased in high-passage, lineage-restricted cultures.
Figure 1 demonstrates an example of k LC-restricted CBLs with the following surface phenotypic marker expression profile: [CD34.sup.+] (partial), [CD44.sup.+], HLA-[DR.sup.+], CD31 (partial), CD184
, CD45RO (partial), [CD50.sup.+], [CD10.sup.-], [CD11b.sup.-], [CD14.sup.-], [CD19.sup.-], [CD20.sup.-], [CD49d.sup.-], [CD54.sup.-], [CD56.sup.-], [CD62L.sup.-], [CD117.sup.-], [CD126.sup.-], [CD130.sup.-], and [CD138.sup.-].
The phenotypic characteristics of ADRCs from the SVF were analyzed by flow cytometry in a subset of 15 sequentially enrolled patients (CD34: 70.4% (range 66.5-73.3), CD45: 21.9% (range 17.3-26.0), CD184
: 13.8% (range 6.9-17.1), vascular endothelial growth factor receptors: 10.8% (range 6.7-17.3), CD31: 10.3% (range 8.7-14.5), CD71: 2.8% (range 1.3-5.7), and CD105: 1.7% (range 0.6-2.6)).
[10.sup.6] cells were incubated with the following conjugated antibodies against human cell surface molecules: CD29, CD34 (hematopoietic stem/progenitor cells/endothelium), CD44, CD45, CD73, CD90 (common leukocyte antigens), HLA-DR (human leukocyte antigen, class II), CD14 (monocyte/macrophage), CD184
and STRO-1 (Stromal Cell Surface Marker) (PharMingen-BD Biosciences, USA), conjugated with FITC (Santacruz, USA) or PE (PharMingen-BD Biosciences).
Comparably to their minimal expression profiles observed in hepatocytes, the cell surface receptors CD45, CD95, CD184
, CD200R, CD210, CD229, and CD271 showed a very weak, if any, expression in undifferentiated as well as hepatic-differentiated ADHLSCs.
In this study, we used flow cytometry to analyse the effects of short-term hypoxic stress on the expression of CD29 (integrin [beta]1), CD44 (HCAM), CD49d (integrin [alpha]4), CD49e (integrin [alpha]5), CD51 (integrin aV), CD61 (integrin [beta]3), and CD184
The surface expression of CXCR4 and CXCR7 on CB-derived MSC was examined using PE-conjugated antihuman CD184
(CXCR4, clone 12G5, BD Biosciences Mississauga, ON, Canada) and PE-conjugated antihuman CXCR7 (clone 11G8, R&D Systems, Minneapolis, MN, USA).