Qin, "Elevated circulating VE-cadherin + CD144
+ endothelial microparticles in ischemic cerebrovascular disease," Thrombosis Research, vol.
MSCs do not express CD31, CD144
, and CD309 (endothelial cell markers) or CD14, CD34, CD45, CD117, and CD133 (hematopoietic cell markers) [61, 79].
Endothelial EVs (CD144
, Annexin [V.sup.+ve]) increase production of superoxide anion and hydrogen peroxide in cultured endothelial cells through NADPH oxidase and mitochondria [38, 39], although others suggest that xanthine oxidase may contribute in endothelial (CD144-PE) , lymphocytic (CD4, CD3+, CD8, CD11a, Fas, and FasL) , and monocyte-derived EVs .
EMVs' surface contains many antigen epitopes; CD144
is one of the most commonly used antibodies for identification of EMVs .
After four hours of low-tide MV, plasma EMP levels were significantly increased in both the C57BL/6 and TLR4KO mice (P < 0.01 for CD144
, P < 0.01 for CD62E, P < 0.01 for CD31, P < 0.05 for CD51, and P < 0.05 for CD54).
Then, the cells were incubated with FITC-conjugated anti-CD44 and CD144
, phycoerythrin-conjugated anti-CD34 and CD106, and PerCP-conjugated anti-CD105 antibodies (all from Abcam, Cambridge, UK) for 30 min.
After blocking with 5% bovine serum albumin, cells were incubated overnight at 4[degrees]C with specific primary antibody, which was one of CD31 (PECAM-1), CD54 (ICAM-1), CD106 (VCAM-1) and CD144
In characterising ADSCs, they phenotypically identify as mesenchymal stem cells by expressing well documented cluster of differentiation (CD) markers, including CD13, CD29, CD31, CD34, CD44, CD63, CD73, CD90, and CD144
For analysis of endothelial progenitor cells (EPCs), fresh whole blood EDTA-anticoagulated samples (100 [micro]L) were incubated for 30 minutes at room temperature with the following monoclonal antibodies: 20 [micro]L of FITC anti-human CD34, 5 [micro]L of PE anti-human CD144
(VE-cadherin), or 5 [micro]L of PE antihuman CD309 (VEGFR-2) (BD PharMingen, Erembodegen, Belgium).
We incubated 10 [micro]L EMP suspension with 10 [micro]L antihuman/rat E-selectin, CD144
, CD31, and CD42 or isotype control.
Tumor cells stain for the usual vascular and endothelial markers including CD31, CD34, CD144
(VE-cadherin), and at least focally for factor VIII-related antigen.
Cells were positive for endothelial marker CD31 (96.17% [+ or -] 1.03%), CD144
(VE-Cadherin, 96.77% [+ or -] 0.37%), and KDR (VEGFR2, 60.67% [+ or -] 14.03%) and were negative for the hematopoietic-related antigen CD45 (1.57% [+ or -] 0.40%) and monocyte differentiation antigen CD14 (1.23% [+ or -] 0.26%).