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Then, the cells were incubated with FITC-conjugated anti-CD44 and CD144, phycoerythrin-conjugated anti-CD34 and CD106, and PerCP-conjugated anti-CD105 antibodies (all from Abcam, Cambridge, UK) for 30 min.
After blocking with 5% bovine serum albumin, cells were incubated overnight at 4[degrees]C with specific primary antibody, which was one of CD31 (PECAM-1), CD54 (ICAM-1), CD106 (VCAM-1) and CD144 (VE-cadherin).
We incubated 10 [micro]L EMP suspension with 10 [micro]L antihuman/rat E-selectin, CD144, CD31, and CD42 or isotype control.
Tumor cells stain for the usual vascular and endothelial markers including CD31, CD34, CD144 (VE-cadherin), and at least focally for factor VIII-related antigen.
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- CD11b antigen
- CD121a antigen
- CD124 antigen
- CD132 antigen
- CD135 antigen
- CD14 antigen
- CD140 antigen-like family member A
- CD140 antigen-like family member B
- CD140a antigen
- CD140b antigen
- CD144 antigen
- CD154 antigen
- CD156a Antigen
- CD156b Antigen
- CD156c Antigen
- CD157 antigen
- CD161 antigen
- CD167 antigen-like family member A
- CD167 antigen-like family member B
- CD167a antigen
- CD167b antigen
- CD172 antigen-like family member A
- CD172 antigen-like family member B
- CD172a antigen