The nonclassical MSC markers were identified in adipose-derived MSCs (AMSCs) grown in human platelet lysate (hPL) culture medium  and included CD163, CD271, CD200, CD36, CD274, CD146, CD248, and CD140b. As shown in Figure 3 and Table 3, CD200 was the only marker that was strongly expressed in the pMSCs from all of the five randomly selected donors (Table 1, donor #6 to donor #10), and there were some weak expressions of CD274 and CD140b in donor #6 (Figure 3(a)) and CD274 and CD146 in donor #10 (Figure 3(b)).
 have reported that the AMSCs positively express CD36, CD146, CD248, CD140b, and CD274 and lack of expression of CD163, CD271, and CD200, but in the present study, pMSCs are CD200-positive and CD248-negative (Table 3).
To clarify protein phosphorylation, [beta]-catenin, [beta]-catenin (pS45), Akt (pT308), Akt (pS473), CD140b, Stat1, Stat3, Stat4, Stat5, Stat6, p38 MAPK, Smad2/3, and ERK1/2 were measured using their respective antibodies.
[beta]-catenin, [beta]-catenin (pS45), Akt (pS473), Stat3, Stat4, Stat5, and ERK1/2 were detected, whereas Akt (pT308), CD140b, Stat1, Stat6, p38 MAPK, and Smad2/3 were not detected in mADSCs.
Cells that were CD[44.sup.+], CD[24.sup.low], ES[A.sup.+] and [lineage.sup.-] (cells lacking markers CD2, DC3, CD10, CD16, CD18, CD31, CD64 and CD140b
), isolated from one primary breast cancer and eight malignant pleural effusion samples, were able to form heterogeneous tumours containing both the CD[44.sup.+], CD[24.sup.low], ES[A.sup.+] and [lineage.sup.-] tumour initiating cells and also the phenotypically diverse nontumorigenic cells that comprised the bulk of tumours (see stem cell definition above).
Flow cytometry analysis undertaken on trypsinated cells showed that they expressed CD44, CD54, CD73, CD90, CD105, CD140b (also known as platelet-derived growth factor (PDGF) receptor-[beta]), CD146, and [alpha]SMA, and were negative for HLA-DR, CD31, CD45, CD56 (Figure 1(b)), and CD34 (not shown).
These cells expressed CD44, CD54, CD73, CD90, CD105, CD140b, and CD146, and were negative for HLA-DR, CD31, CD45, CD56 (Figure 4(d)), and CD34 (not shown).
Antibodies used included fluorochrome-conjugated anti-human CD11b, CD31, CD34, CD79a, CD90, CD105, CD106, CD146, CD271, CD140b, and NG2 immunoglobulins.
Histological analysis of AT indicates that cells in the tunica adventitia and endothelium of arteries and arterioles express CD34; the layer between the adventitia and endothelium contains cells that simultaneously express [alpha]-SMA and CD140b  or [alpha]-SMA and CD146 [55, 57].
Interestingly, ASCs in their native microenvironment were negative for CD140b
and NG2 pericyte markers but the expression of them was induced by culture process .
These cells also express typical stromal markers (CD105, CD49a, CD73, CD90, and CD140b
) and are capable of robust trilineage differentiation.
Subsets of these cells also express NG2 chondroitin sulfate proteoglycan and CD140b
(platelet-derived growth factor receptor B), which have been associated with pericytes/perivascular cells and MSCs in many tissues .