CM were collected from H2O2 treated MSCs (acutely senescent cells) following transfection with Filamin 3 siRNA or CD13
siRNA or control siRNA, respectively.
was studied among the blast population with mean positivity of 57%+-27.53 among all subtypes with strongest positivity in AML-M4 (72% +- 20.01) and weakest in AML-M6 (22% +- 1.73).
Taken together, these results demonstrate that selected combinations of inflammatory cytokines, along with the promotion of the survival of CB-derived [CD34.sup.+] cells, stimulate the expression of CD13
, which is an early and late myeloid marker.
Metcalfe, "Demonstration that human mast cells arise from a progenitor cell population that is [CD34.sup.+], [c-kit.sup.+], and expresses aminopeptidase N (CD13
)," Blood, vol.
was proven to be functionally significant in the maintenance of cancer stem cells.
Cyclic NGR (i.e., CNGRCVSGCAGRC) is a ligand for CD13
, which is upregulated primarily in the tumor vasculature and in a few cancers such as fibrosarcomas.
However, in competition studies, the RGD motif was found to have a lower target homing ratio (target to control tissue) compared to the Asn-Gly-Arg (NGR) tripeptide sequence which binds to selectively upregulated CD13
on angiogenically active endothelial cells .
EpCAM and CD13
are some stemness-related markers in HCC [11, 12], and several studies have demonstrated that EpCAM or CD13
expression in HCC tumor tissue is associated with a poor prognosis [13, 14].
Bone marrow biopsy and aspirate showed 73% myeloblasts and immature promonocytes expressing CD13
, CD15, CD33, CD45, CD64, CD123, and HLA-DR by immunohistochemistry (IHC) and flow cytometry (FC).
(GP150; aminopeptidase-N): predominant functional activity in blood is localized to plasma and is not cell-surface associated," Experimental Hematology, vol.
(BA0718), CD44 (BA0321), CD106 (BA0406), and CD49d (BA1640) antibodies were from Boster Biological (Wuhan, China).
The primary antibodies used were CD13
, CD90, CD73, CD45, CD34, CD38, CD105, CD54, and CD44.