Kabelitz, "Differential expression of CD126
and CD130 mediates different STAT-3 phosphorylation in CD4+CD25- and CD25high regulatory T cells," International Immunology, vol.
For detection of IL-6 and IFN-[gamma] receptors, 400,000 ASC (n = 3) were stained for two IL-6 receptor subunits (CD126 and CD130) and IFN-[gamma] receptor (CD119).
To examine whether ASC-secreted IL-6 has an autocrine function, we measured the expression of the IL-6 receptor [alpha]-chain (CD126) and the IL-6 receptor [beta]-chain (CD130) by flow cytometry (Figure 4).
IL-6 exerts its activity through interaction with a receptor complex composed of the nonsignaling alpha subunit IL6R (CD126
) and the common, ubiquitously expressed, beta subunit gp130 (CD130), resulting in immediate activation of receptor-associated kinases (JAK1/JAK2 and TYK2) and subsequent regulation of STAT1/STAT3 and SHP2-MAPK signaling pathways (Figure 1) 68, 70, 71].
FITC PE PE-Cy5 APC 1 [kappa] CD34 CD62L CD38 2 [kappa] CD14 CD44 CD38 3 [kappa] CD126
HLA-DR CD38 4 [kappa] CD117 CD128 CD38 5 [kappa] CD11b CD38 CD3 6 [kappa] CD184 CD38 CD19 7 [kappa] CD45ro CD38 CD10 8 [kappa] CD31 CD38 CD54 9 [kappa] CD50 CD38 CD49d 10 [kappa] CD130 CD38 CD44 11 [kappa] CD56 CD38 CD20 12 [lambda] CD34 CD62L CD38 13 [lambda] CD14 CD44 CD38 14 [lambda] CD126
HLA-DR CD38 15 [lambda] CD117 CD128 CD38 16 [lambda] CD11b CD38 CD3 17 [lambda] CD184 CD38 CD19 18 [lambda] CD45ro CD38 CD10 19 [lambda] CD31 CD38 CD54 20 [lambda] CD50 CD38 CD49d 21 [lambda] CD130 CD38 CD44 22 [lambda] CD56 CD38 CD20 Abbreviations: APC, allophycocyanin; FITC, fluorescein isothiocyanate; PE, phycoerythrin; PE-Cy5, phycoerythrin-Cy5.
IL-6 binds to its receptor IL-6R, known as CD126
, leading to activation of receptor-associated kinases, for example, Janus kinase 2 (JAK2) (Additional File 17B) .
There are two subtypes of the IL-6R receptor (gp80, CD126
), namely, the membrane form of mIL-6R and the soluble sIL-6R, which maybe formed by cutting or by the ADAM17/TACE by metalloproteinase or by means of alternative splicing .