Flow cytometric analysis revealed that these MSCs were positive for CD29, CD71, CD73 (SH3), CD90, CD105 (SH2), CD106, CD144, CD120a
, and CD 124 [69-72], while they were negative for CD34, CD31, VEGFR2, CD62E, vWF, VE-cadherin, VCAM-1, and ICAM-1 [69-72].
The human protein encoded by the TNFRSF1A gene is one of the major receptors for TNF-[alpha], and TNFR1 (CD120a
) can be detected in almost all cell types, including endothelial cells [26, 27].
Several biological markers characterize MSCs of different origins to be positive for CD73, CD105, CD29, CD44, CD71, CD90, CD106, CD120a
, and CD124 and negative for CD117, CD34, CD45, and CD14 [19-21].
Scheurich, "The type 1 receptor (CD120a
) is the high-affinity receptor for soluble tumor necrosis factor," Proceedings of the National Academy of Sciences of the United States of America, vol.
The cytokine has the ability to bind to the two isotypes of membrane receptors located on the surface of almost every nucleated cell TNF-R1 (p55, CD120a
, and TNFRSF1a) and TNF-R2 (p75, CD120b, and TNFRSF1b) [52, 53].
TNF[alpha] is capable of eliciting a variety of different immune responses by signalling via two types of membrane-bound receptors, type I (CD120a
, TNFRSF1A) and type II (CD120b, TNFRSF1B) receptors, with respective molecular weights of 55 and 75kDa [5, 6].