They may be negative for CD5, CD10 and CD23 and positive or negative for CD43 and CD11c
At least one histiocytic or histiocyte-associated marker (CD163, CD68, CD11c
, lysozyme) will be positive in cases of histiocytic sarcoma.
After 15 min of incubation, flow cytometric analysis was performed to detect migrating macrophages (CD11b + CD11C
−), macrophages in situ (CD11b − CD11C
+), and macrophage debris (CD11b − CD11C
Khurram et al10 found 26% cases aberrantly expressed either of CD7, CD19, CD4, CD2, CD13, CD117, CD11c
Among chronic leukemia, all aberrant expressions were seen in cases of B-CLL (10/28) only; with CD11c
, CD3 and CD10 as the aberrantly expressed markers.
With suspicion of LAD it was decided to investigate it and diagnosis was confirmed by flow cytometry showing absence of CD18, CD11b, CD11c
Since both mDC and pDC are CD11c
positive [14-16], CD11c
is commonly used as a murine DC marker along with costimulator molecules like MHC II, CD40, CD80, and CD86 .
To compare the effects of these two types of MSCs on the composition and activation of lymphocytes infiltrated into SMGs, we examined the expression of various lymphocyte markers including B cell marker CD19; B/plasma cell markers CD79a, CD79b, and Ighg3; pan-T cell marker CD3e; helper T cell marker CD4; Treg marker Foxp3; Tfh marker inducible T cell costimulator (Icos) and its ligand Icosl; Th17 marker IL17; panAPC marker CD11c
; follicular dendritic cell (FDC) marker CD21; and activated APC marker CD40, as well as those of immune inhibitory factors including TGF[beta]1-3 and IL10 by qRT-PCR.
We also used CD11c
(clone N418; FITC) and MHC-II (clone AF6-120.1; APC) from Thermo Fisher.
The cells also typically express CD103, CD11c
, and CD25 but usually not CD5, CD10, or CD23.
In addition, sitagliptin significantly decreased the mRNA expressions of Cd68, F4/80, and M1 marker (Cd11c
) and increased the M2 marker Cd206, showing the reduced number of infiltrated macrophages and attenuated M1/M2 polarization imbalance in the liver (Figure 4(c), p < 0.01).
The following primers were utilized: CD11c
, sense 5'-GTGCCCATCAGTTCCT TACA-3' and antisense 5'-GAGAAGAACTGTGGAGCTG AC-3'; CD206, sense 5'-GGAATCAAGGGCACAGAGT TA-3' and antisense 5'-ATTGTGGAGCAGATGGAA-3; F4/80, sense 5'-CGTCAGGTACGGGATGAATATAAG-3' and antisense 5 ' -CTATGCCATCCACTTCCAAGAT-3'; and cyclophilin A, sense 5' -CAGACGCCACTGTCGCTTT3' and antisense 5'-TGTCTTTGGAACTTTGTCTGCAA-3'.