Xiao et al., "Hypomethylation and overexpression of ITGAL (CD11a
) in [CD4.sup.+] T cells in systemic sclerosis," Clinical Epigenetics, vol.
The OCT-embedded sections were fixed in methanol, washed, and incubated with fluorescein isothiocyanate conjugated (FITC) rat anti-mouse CD11a
antibody 1 : 250 (SouthernBiotech) for 2 hours in a humidified chamber.
Endothelial EVs (CD144, Annexin [V.sup.+ve]) increase production of superoxide anion and hydrogen peroxide in cultured endothelial cells through NADPH oxidase and mitochondria [38, 39], although others suggest that xanthine oxidase may contribute in endothelial (CD144-PE) , lymphocytic (CD4, CD3+, CD8, CD11a
, Fas, and FasL) , and monocyte-derived EVs .
Role of CD11a
and CD11b in ischemic acute renal failure in rats.
Expressions of CD11a
on [CD4.sup.+] cells in lung and spleen were suppressed following PA-824 and/or CS treatment
Therefore, H3ac is increased and H3K9me3 is decreased in the promoters of CD11a
and CD70 in SLE [CD4.sup.+] T cells, resulting in CD11a
and CD70 overexpression and autoimmune responses.
The following antibodies were used as follow: For flow cytometry determination, CD3, CD8, CD54 (intercellular adhesion molecule [ICAM]-1), CD106 (vascular adhesion molecule [VCAM]-1), CD11a
(lymphocyte function-associated antigen-1 [LFA-1]), CD29 (integrin [sz]1), IL-27, IFN-a, IL-17, phosphorylated (p)-STAT1 (pY701), p-STAT1 (pS727), p-STAT2 (pY689), p-STAT3 (pS727), p-STAT3 (pY705), p-STAT4 (pY693), p-STAT5 (pY694), p-STAT6 (pY641), TCCR-WSX-1 (IL-27 receptor), IL-17 receptor (R), IFN-aR1, and Ki-67 mAbs were purchased from BD Biosciences (Franklin Lakes, USA), eBioscience (San Diego, USA), or R and D systems (Minneapolis, USA); for cytokine neutralization, IL-27, IFN-a, IL-17, ICAM-1 and VCAM-1 mAbs were from eBioscience.
(a) System Antigens CD Gene cDNA sequence HNA-1 HNA-1a CD16 FCGR3B (b) NM_000570.4 HNA-1b HNA-1c HNA-1d HNA-2 HNA-2a CD177 CD177 NM_020406.2 HNA-3 HNA-3a CTL2 (c) SLC44A2 NM_020428.3 HNA-3b HNA-4 HNA-4a CD11a
ITGAM NM_001145808.1 HNA-4b HNA-5 HNA-5a CD11b ITGAL NM_001114380.1 HNA-5b (a) Veldhuisen et al.
Efalizumab is a monoclonal antibody against CD11a
. Efalizumab is FDA approved to treat psoriasis.
Efalizumab is a recombinant humanised monoclonal IgG1 antibody that binds to CD11a
, a subunit of leukocyte function-associated antigen 1 (LFA-1) (24).
As [beta]2-integrinas (CD11a
, b, c/CD18) tambem sao moleculas de adesao da superficie celular envolvidas na migracao de neutrofilos (SMITS et al, 2000; RAINARD; RIOLLET, 2003; 2006).
These cells are characterized by the surface pattern of effector-type or effector-memory T cells (expression of CD45RA, CD11a
, CD11b, CLA, [alpha]E[beta]7 and absence of CD27, CD28, CD56, CD62L).