Several studies have highlighted the significance of inflammation as an early event after stenting in different animal models using specific antibodies to leukocytes (CD45), macrophages (CD14), and monocytes (CD115
). The anti-inflammatory effect of DXM was by inhibiting lipopolysaccharide (LPS)-induced superoxide production than the production of nitric oxide and tumor necrosis factor-a (TNF-a).
Another characteristic of activated M2 monocytes is increased expression of CD206 and CD115
Bone marrow [CD115.sup.+] monocytes were sorted by a CD115
microbead kit (Miltenyi Biotec, Bergisch Gladbach, Germany, catalog number 130-096-354) according to the manufacturer's instructions.
It also became clear that monocytes express RANK and c-fms (CD115
), and the presence of their ligands RANKL and M-CSF, respectively, was necessary and sufficient for osteoclastogenesis to occur [8, 14, 15].
Introduction of additional markers allowed for further characterization of these subsets indicating that CD14lowCD16+ monocytes expressed higher levels of CD43, CD115
, CXCL16, CXCR1 and C3AR1 expression whereas CD14highCD16- monocytes exhibited higher levels of CD1d, CD93 and CD114.
Phycoerythrin- (PE-) labeled anti-mouse F4/80 antibodies, allophycocyanin (APC)/Alexa Fluor 750-labeled anti-mouse CD11b antibodies, and APC-labeled anti-mouse CD115
antibodies in 25 [micro]L of cell staining buffer were added and allowed to react in darkness at 4[degrees]C for 15 minutes to identify the mononuclear leukocytes in the HMNC suspension.
Todas mis Esperansas, 1940s Recordings of Judeo-Spanish, Greek and Turkish Song, New York, Global Village CD115
IL-34 is a newly discovered cytokine, which is consisted of 222 amino acids and shares the same receptor with macrophage colony-stimulating factor (M-CSF) [4, 5], also called colony-stimulating factor-1 receptor (CSF-1R or CD115
Then, the cells were stained with antibodies to CD34 (Santa Cruz Biotechnology, USA), VEGFR-1 (Sigma-Aldrich, USA), CD115
, CD117 (Abcam, USA), CD14, CCR2 (Santa Cruz Biotechnology), and CD90, CD44, STRO-1, OCT3/4, and Nanog, all from Abcam, at a concentration of 1 [micro]g/mL at 4[degrees]C for 30 min.
Cell suspensions were stained with the following appropriately labeled antibodies: CD11b, Ly6G- Ly6C, CD4, CD25, Foxp3, mPDCA-1, CD11c, CD115
, MHC-II, CD80, and CD86 (BD Biosciences, Pont de-Claix, France).
As these [MHCII.sup.Int] [CD11b.sup.+] [CD11c.sup.+] have been referred to as more macrophage-like, we measured two surface markers: CD115
, also known as M-CSF receptor, and MERTK.
It has been demonstrated that cells that in Mtb-infected mice fall into the "[Gr-1/Ly-6G.sup.hi]" gates (TBAN) differ from [Gr-1/Ly-6G.sup.hi] neutrophils found in steady-state conditions ("steady-state neutrophils" (SSN)): TBAN had lower expression levels of Gr-1/Ly-6G, elevated expression of immaturity markers CD115
and CD135, and, in contrast to SSN, were able to inhibit T cell proliferation .