As shown in Figure 1A, at passage 2, purified GMSC's expressed significant levels of CD106
and CD44 (p<0.01) (sternness factors) and CD13 and CD34 (p<0.05) (specific markers for satellite cells).
The P3 cells were then detected in the surface antigens (CD29, CD45, CD44, and CD106
, Biolegend) using FACScan flow cytometer (BD FASCanto[TM], USA).
The [CD45.sup.-] SP subpopulation was enriched with cells expressing the ABCG2 gene (along with other transcripts such as CD31, CD106
, CD133, and endoglin) and showed greater efflux capacity when compared to the [CD45.sup.+] SP subpopulation .
EV origin Surface markers Endothelium [CD31.sup.+]/[CD41.sup.-]([PECAM.sup.+]/[ITGA2B.sup.-]) [CD31.sup.+]/[CD42.sup.-] ([PECAM.sup.+]/[GPIb.sup.-]) CD31 (PECAM (platelet cell adhesion molecule)) CD144 (VE cadherin (vascular endothelial cadherin)) CD146 (MCAM (melanoma cell adhesion molecule)) CD105 (endoglin) CD106
(VCAM (vascular cell adhesion molecule)) CD62E (E-selectin (endothelial-selectin)) Platelet CD41 (ITGA2B (integrin alpha 2b)) CD42 (GPIb (glycoprotein Ib)) CD31 (PECAM (platelet cell adhesion molecule)) Leukocyte CD45 (PTPRC (protein tyrosine phosphate receptor type C)) CD11b (ITGAM (integrin alpha M)) CD14 (coreceptor of lipopolysaccharide) CD16 (on surface of neutrophils, monocytes, and macrophages) CD62L (L-selectin (leukocyte selectin)) Red blood cell CD235 (glycophorin A)
In addition, pECs express the following adhesion molecules: PECAM-1 (CD31), E-selectin (CD62E), P-selectin (CD62P), and vascular cell adhesion molecule-1 (VCAM-1, CD106
) [52, 53].
Cells experimentally exposed to [alpha]-solanine expressed high levels of the CD90, CD29, CD71, and CD106
stem cell markers.
ASCs can be characterized while in culture dish as [CD73.sup.+]/[CD90.sup.+]/[CD105.sup.+]/[CD44.sup.+]/[CD45.sup.-]/[CD31.sup.-] cells, which can be distinguished from the bone marrow-derived MSCs by their expressions of CD36 and negative for CD106
molecules on their cell surface .
The stem cell functionality of hDPSCs was confirmed by positive expression (over 90%) of CD73, CD105, and CD106
, and negative expression (less than 1%) of CD34 and CD45 by flow cytometry (data not shown).
Then, the cells were incubated with FITC-conjugated anti-CD44 and CD144, phycoerythrin-conjugated anti-CD34 and CD106
, and PerCP-conjugated anti-CD105 antibodies (all from Abcam, Cambridge, UK) for 30 min.
Some embryonic and mesenchymal stem cell markers antibodies such as Nanog, OCT4 (Abcam ab19857 1:500), SOX2, CD44 (Abcam ab119863 1:500), CD105 (Abcam ab44967 1:250), CD106
(Abcam ab19569 1:500), CD90 (Abcam ab2251: 250), and CD133 (Millipore MAB4399) were used to characterize the isolated cells from human breast milk by immunocytochemistry method.
A substantial body of evidence suggests that a large number of mediators, including cell adhesion molecules such as intercellular adhesion molecule-1 (ICAM-1, CD54) and vascular cell adhesion molecule-1 (VCAM-1, CD106
)  as well as proinflammatory cytokines such as TNF-[alpha],IL1,IL6, and IL8, and chemokines such as MCP-1, play key roles in thepathogenesis of endometriosis.
Alternate theories of the mechanism of sequestration of IVLBCL to the intravascular space include deficient expression of receptors for peanut agglutinin and CD49d, both of which promote homing of tumor cells to endothelial cells via interaction with CD54 and CD106