Then, the cells were incubated with FITC-conjugated anti-CD44 and CD144, phycoerythrin-conjugated anti-CD34 and CD106
, and PerCP-conjugated anti-CD105 antibodies (all from Abcam, Cambridge, UK) for 30 min.
BMSCs express a number of nonspecific markers as CD105 (SH2), CD73 (SH3/4), CD44, CD90 (Thy-1), CD71, and Stro-1, as well as the adhesion molecules CD106
and CD166, intercellular adhesion molecule1, and CD29 .
The following antibodies were used as follow: For flow cytometry determination, CD3, CD8, CD54 (intercellular adhesion molecule [ICAM]-1), CD106
(vascular adhesion molecule [VCAM]-1), CD11a (lymphocyte function-associated antigen-1 [LFA-1]), CD29 (integrin [sz]1), IL-27, IFN-a, IL-17, phosphorylated (p)-STAT1 (pY701), p-STAT1 (pS727), p-STAT2 (pY689), p-STAT3 (pS727), p-STAT3 (pY705), p-STAT4 (pY693), p-STAT5 (pY694), p-STAT6 (pY641), TCCR-WSX-1 (IL-27 receptor), IL-17 receptor (R), IFN-aR1, and Ki-67 mAbs were purchased from BD Biosciences (Franklin Lakes, USA), eBioscience (San Diego, USA), or R and D systems (Minneapolis, USA); for cytokine neutralization, IL-27, IFN-a, IL-17, ICAM-1 and VCAM-1 mAbs were from eBioscience.
Alternate theories of the mechanism of sequestration of IVLBCL to the intravascular space include deficient expression of receptors for peanut agglutinin and CD49d, both of which promote homing of tumor cells to endothelial cells via interaction with CD54 and CD106
Mesenquimales (estromales): CD29, CD44, CD71, CD90 y CD106
A populacao de MSC isoladas da medula ossea de humanos e camundongos expressa em sua superficie marcadores moleculares como: CD44 (receptor de hialuronato), CD105 (endoglina: marcador angiogenico), CD106
(VCAM-1: molecula de adesao vascular), CD166 (ALCAM: moleculas de adesao de leucocitos ativados), CD29 (integrinas VLA-[beta]), CD73 (SH3 e SH4), CD90 (Thy-1), Stro-1(estroma de suporte da hematopoese) e Sca-1 (GRONTHOS et al.
This study was undertaken to examine the tissue distribution of a broad spectrum of monoclonal antibodies (mAbs) containing tetraspan antigens (CD9, CD63, CD151), endothelial cell antigens (CD31, CD36, CD105, CD106, CD146), integrins (CD49a-f, CD29, CD41, CD51, CD61), CD39, CD98, CD99, CD143 and, CD147 supplied from fifth and sixth international workshops and conferences on human leukocyte differentiation antigens in a comparative manner in human OA and normal synovium.
CD106 exhibited a moderate to strong immunoreactivity with the SLL; the reaction shifted significantly onto fibroblast-like type B synoviocytes located at the upper layers in the OA group (p < 0.
After blocking with 5% bovine serum albumin, cells were incubated overnight at 4[degrees]C with specific primary antibody, which was one of CD31 (PECAM-1), CD54 (ICAM-1), CD106
(VCAM-1) and CD144 (VE-cadherin).
The NF-[kappa]B inhibitor, tepoxalin, suppresses surface expression of the cell adhesion molecules CD62E, CD11b/CD18 and CD106