HHDPC are characterized by their mesenchymal cell morphology and immunofluorescent method with antibody to fibronectin and CD105
The hUDSCs were harvested and ThehUDSCs were harvested and labeled with antibodies against CD45, CD34, CD90, CD105
, CD133, and CD44.
The human renal carcinoma cells expressing CD105
release microvesicles that stimulate neoangiogenesis.
MSCs possess the following traits: plastic adherent properties; positivity for the cell surface markers CD105
, CD90, and CD44; and an ability to differentiate into a mesodermal lineage (osteogenic, alipogenic, and chondrogenic cells) (3).
RT-PCR primers for pluripotency (Oct3/4, Nanog, Sox2), MSC surface markers (CD4, CD8A, CD25, CD33, CD34, CD44, CD54, CD61, CD80, CD105
, CD117, Flk-1 and HMGA2) and GAPDH internal control were shown in
Minimal criteria for defining MSC according to International Society of Cell Therapy (ISCT) are (a) they must be plastic-adherent when maintained in a standard culture conditions, (b) MSC must express some surface antigen such as CD105
, CD73, CD90, CD44 and must not express CD34, CD45, CD 14, CD19, and HLA-DR, and (c) MSC must differentiate in vitro into osteoblast, adipocytes and chondrocytes under specific differentiating condition (4).
The flow cytometric analysis showed that isolated cells were positive for CD146, CD90, CD105
and were negative for CD31, CD34 (Figure 2).
After excluding cell debris with a morphological gate, we stained cells with fluorescent-tagged antibodies to CD34, CD146, and CD105
and matched isotype controls.
Density of microvessels positive for CD105
(endoglin) is related to prognosis in meningiomas.
We also detected CD29, CD105
and CD133 gene expression by RT-PCR as markers for MSCs .
TRC105 is TRACON's monoclonal antibody to CD105
, an endothelial cell membrane receptor that is essential for the process of new blood vessel formation called angiogenesis.
Antigen CD29 CD44 CD90 CD105
CDI66 CD31 CD16 Content (%) 98.