The explant derived MSCs were analysed at 3rd Passage which expresses positive markers CD73, CD105
, CD90 and CD44 and lacks expression of negative MSC cocktail (PE CD45, PE CD34, PE CD11b, PE CD19 and PE HLA-DR) of all the cells population can be observed (Fig.
BMSCs were analyzed by fluorescence immunoassay to detect the surface markers (CD34, CD44, CD105
Primary antibodies against CD271, CD90, CD146, CD44, CD73, and CD105
obtained from BD were analyzed using Navios (Beckman Coulter, USA).
Surface marker Oct4, CD105
, nanog, CD73 and SSEA-4 on CAFSCs are detected positive with immunofluorescence histochemistry, Gene CD44, CD29, CD73 and SH2 are detected positive with RT-PCR.
MenSCs and BMSCs were harvested after two passages and evaluated for their immunophenotype characteristics using antibodies against MSCs markers: CD9, CD 10, CD44, CD73 and CD105
, embryonic stem cells markers; Oct-4, Nanog, Stro-1 and SSEA-4 and hematopoietic markers; CD34, CD38, CD45, and CD 133.
Immunohistochemical Comparison of the Expression of CD34 and CD105
in Odontogenic Keratocyst and Dentigerous Cyst.
(endoglin) staining is highly expressed only in angiosarcoma-associated endothelial cells.
Staining with monoclonal antibodies (PerCP-Cy5.5 mouse antihuman CD105
, APC mouse antihuman CD73, FITC mouse antihuman CD90, PE-Cy5 mouse antihuman HLA-DR, APC mouse antihuman CD34, and FITC mouse antihuman CD45) in accordance with manufacturer's instructions (BD Pharmingen, BD Horizon USA).
Among those proangiogenic biomarkers, endoglin (CD105
) has been shown to be remarkably upregulated on highly proliferating endothelial cells (neovasculature wall), instead of the normal endothelial cells or mature vessels [5, 6].
The expressions of hBM-MSC surface markers cluster of differentiation (CD14, CD34, CD45, CD90, CD73, and CD105
) were detected by BD FACS Calibur[TM] (BD Biosciences, San Jose, CA, USA), and cells were characterized as having high expression of these positive markers (CD90, CD73, and CD105
) and low expression of the negative markers (CD14, CD34, and CD45) .
A multitude of immunoreactive studies maybe performed including S-100, CD34, CD68, CD105
, and many others.