Two-way ANOVA was applied for comparing CD1 and C57BL/6 mice at different time points.
CD1 Mice Display Increased Susceptibility to NTS Compared to C57BL/6 Mice.
Based on these results, 250 [micro]l and 100 [micro]l NTS were selected for subsequent experiments in C57BL/6 and CD1, respectively.
The CD1 NTN mice developed significantly increased UAER on days 2-3, 6-7, and 16-17 compared to C57BL/6 NTN mice (Figure 3(a)).
We previously reported that the CD1 gene is frequently amplified in both the components of esophageal carcinosarcoma.[5,6] As esophageal carcinosarcoma has the same morphologic and immunohistochemical features as carcinosarcoma of the tongue, CD1 gene amplification may be a common important event in the molecular pathogenesis of tumors of this morphologic type.
Recently, Nogueira et al reported that CD1 gene amplification is associated with recurrence and/or metachronous tumors in head and neck squamous cell carcinoma, and Mineta et al reported that CD1 overexpression correlates with poor prognosis in patients with tongue squamous cell carcinoma.
CD1 shares many structural features with MHC class I molecules.
Similarly to MHC class I, CD1 molecules possess two deep pockets: A' and F'.
After translation, CD1 molecules initiate their maturation process in the endoplasmic reticulum (ER).
For immunization C57BL/6 and CD1 mice were injected subcutaneously on day 0 with 200 [micro]L of an emulsion containing 300 [micro]g of [MOG.sub.35-55] (CNB, CSIC, Madrid, Spain) and 800 [micro]g of heat killed Mycobacterium tuberculosis H37RA (Difco, BD Diagnostics, MD) emulsified in incomplete Freund adjuvant oil (Sigma-Aldrich, St.
We explored regulatory and effector immune mechanisms in CD1 and C57BL/6 mice immunized with [MOG.sub.35-55], using as controls nonimmunized mice and mice immunized with [PLP.sub.139-151], a myelin peptide that is not capable of inducing EAE in any of those mouse strains.
Moreover, upon antigen-specific stimulation, the splenocytes from CD1 resistant mice did not proliferate, while those of C57BL/6 susceptible mice did (P = 0.016, Figure 1).