Koets, "[gamma][delta] T cell homing to skin and migration to skin-draining lymph nodes is
CCR7 independent," The Journal of Immunology, vol.
The complete
CCR7 open reading frame (ORF) was PCR-amplified with primers containing ApaI or NotI restriction sites (57-agggggcccgccaccATGGACCCAGGGAAACCCAGG-37 and 57-ataagaatgcggccgcCTACGGGGAGAAGGTTGTGGTG-37, resp.) and inserted into the pDC316mCMV-EGFP shuttle vector to generate pDC316-mCMVCCR7-EGFP.
Tfh and Th17 cells also differ in the ability to home to different immune microenvironments; while most Tfh cells are [CXCR5.sup.+] and migrate to the secondary lymphoid tissue B cell areas, Th17 cells, when activated, down regulate
CCR7 and up regulate CCR6, migrating to the target organs where they exert their effector functions.
CCL21, CCL19, and its receptor
CCR7 take part in a series of immunological processes including T cell homeostasis [32], the generation of thy-mocytes [33, 34], regulatory T cell (Treg) function [35-37], and central and peripheral tolerance [38, 39].
However, trogocytosis is characterized by relatively short gene transfer times (72 hours for
CCR7 and 2h for CD19) and has so far relied on liver donor cells to reach high expression levels (from 50% to 80% for
CCR7 and 19% to 47% for CD19).
We also demonstrated that RPE cells express CCR1, CCR3, CCR4, CCR8, and CCR11, while CCR2, CCR5, CCR6, and
CCR7 were not expressed by these cells.
These genes include Ets-1 and FoxP3 [32]; ITGAM and FcyRIIIA [33]; PD-1.3A, C4AQ0, and MBL [34]; AlFadhli (IRF9, ABCA1, APOBEC3, CEACAM3, OSCAR, TNFA1P6, MMP9, and SLC4A1) [35]; FCGR3A and FCGR3B [36]; Tlr7 [37]; TBX21 and IFNG [38]; CD95 and
CCR7 [39]; Fkbp11 [40]; JHDM1D and HDAC1-3 [41]; IL-28RA [42]; andpSTAT1 and ETS1 [43].
The
CCR7 Ligand Is Necessary for the Induction of Pathogenic Th17 Cells.
have suggested that the dendritic cells (DCs) in the decidua are immobile despite being responsive to the chemokine CCL21, one of the ligands for
CCR7 that enable homing to lymphatics.
CCR7 which is expressed by activated T cells [66] as well as innate lymphoid type 3 cells (ILC3) [66, 67] binds both CCL19 and CCL21.
Based on
CCR7 and CD45RA expression, we found that [CD4.sup.+] and [CD8.sup.+] T cells from both compartments were dominated by an effector memory T cell phenotype at the expense of naive cells (Figure 4(a)).