In Syros' study, RB pathway alterations were prospectively defined per The Cancer Genome Atlas criteria including RB1 deletion or mutation, CDKN2A downregulation or deletion, CCNE1
amplification, CCND1 amplification, or CCND2 upregulation.
"Oncogene-driven high-grade serous ovarian cancers, or HGSOC, with CCNE1
and MYCN pathway activation exhibit defective cell cycle checkpoint control and replicative stress.
The data was confirmed by real time PCR for 10 miRNAs (let-7c, miR-10b, miR-26a, miR-26b, miR-29c, miR-30a, miR-29a, miR-29a, miR-30b, miR-30c, miR-148a, and miR-299), and the target genes were subsequently validated (CDC6, CCNE1
, MYBL2, PDCD10, ERBB2IP, SON, STK4, CDC27, PRC1, CDC37, TTK, SKIL, BUB3, and SPIN1).
Amplification of CCNE1
gene encoding cyclin E1 is uncommon in HR+/HER2+ cancer subtype , although overexpression of cyclin E1 is a more common event .
In addition, genes located at the 19q13 are the BAX and BCL3 apoptosis related genes , genes involved in cell cycle progression that codify for cyclins, such as CCNE1
, and genes codifying for transcription factors, such as FOSB , among others .
Shamima et al., "Gene amplification CCNE1
is related to poor survival and potential therapeutic target in ovarian cancer," Cancer, vol.
Knockdown of CDR1AS promoted the expression of miR-7 and suppressed its targets, CCNE1
(cyclin E1) and PIK3CD (phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta): this molecular cascade resulted in a reduction of cell proliferation and invasion in HCC .
miRNA-16 targets several cell cycle regulators, including cyclin D1 (CCND1), cyclin D2 (CCND2), cyclin D3 (CCND3), cyclin E1 (CCNE1
), cyclin-dependent kinase-1 (CDK1), and cyclin-dependent kinase-6 (CDK6).
On the other hand, the interactions from RFC4 to CDC2 and CDC2 to CCNE1
are found not only by BGL and BGL_prior, but also by 2 of other 3 comparable methods.
Jin et al., "Upregulated expression of BCL2, MCM7, and CCNE1
indicate cisplatin-resistance in the set of two human bladder cancer cell lines: T24 cisplatin sensitive and T24R2 cisplatin resistant bladder cancer cell lines," Investigative and Clinical Urology, vol.
When the glucose availability is high and growth factor level is low, the endothelial expression of miR-503 is increased, which causes impairment of endothelial function and angiogenesis via directly inhibiting molecules involved in cell proliferation and survival proteins such as cdc25A and CCNE1