In the cell cycle signaling pathway, we noted alteration in the expression Ccna2
Stag2, Atr, Smc1a and Rbl1 .
For example, the let-7 family has been shown to repress multiple genes involved in cell cycle, cell apoptosis, and cell proliferation, including CCNA2
, CDC34, AURA/STK6, AURKB/ STK12, E2F5, and CDK8 .
It was found that expression of TP53 (HR 1.9, p = 31 x [10.sup.-7]) was associated with worse overall survival (OS) for glioma patients, as well as TOP2A (HR 4.4, p = 0), CDK1 (HR 4.8, p = 0), CCNB1 (HR 5.9, p = 0), CDC20 (HR 5.2, p = 0), CCNA2
(HR 5.1, p = 0), NDC80 (HR 5.8, p = 0), AURKA (HR 5.3, p = 0), BIRC5 (HR 5, p = 0), CCNB2 (HR 5.4, p = 0), KIF11 (HR 2.3, p = 15 x [10.sup.-10]), and MAD2L1 (HR 4.4, p = 0), while expression of PHLPP2 (HR 0.41, p = 1.3x [10.sup.-11]), DLG4 (HR 0.59, p = 35 x [10.sup.-5]), and MYC (HR 0.58, p = 2.1 x [10.sup.-5]) was associated with better overall survival (OS) for glioma patients (Figure 3).
Our data showed that several cell cycle related genes, such as DDIT3, PPP1R15A, and GADD45A, were upregulated in the propofol-treated group, while other genes that are involved in cell cycle progression, including CCNA2
, CDKN3, CDC2, and CDC25B, were downregulated.
Genes included in cluster 4 and 13 were related to cell cycle and renewal of stem cells (CCNB1, CCNA2
, FGF7, MAP2K4, WNT5A, KITGL, and FGF5).
DNMT1 inhibition, DNA hypomethylation, and chromatin remodeling have been shown to facilitate the inhibition of the expression of certain genes, which further mediate Hcy-induced cyclin A (CCNA2
) gene silencing and growth inhibition in regard to ECs [36, 37].
The relative expression of CDK1, CDK2, CCNB1, GTSE1, ITPR1, c-MYC, SRGAP3, HIST2H3D, HIST] H3J, TOP2A, CCNA2
and RHOJ was determined.
Aggressive follicular lymphoma, a subset of NHL, is associated with upregulation of genes involved in cell cycle control such as CCNE2 (cyclin E2), CCNA2
(cyclin A2), CDK2 (cyclin-dependent kinase 2), and genes-reflecting increased metabolism and DNA synthesis .
Other upregulated genes were related to cell proliferation and its control, such as cyclin-dependent kinase gene CDK1/CDC2; cyclin genes CCNA2
, CCNB1, CCNB2, and CCNL2; the DLG7 component of the mitotic apparatus; the gene encoding the checkpoint kinase involved in response to DNA damage, CHEK1/CHK1; and BUB1 and MAD2L1, components of the spindle checkpoint.
In the first case study we end up with the marker genes for (i) the proliferation activity composed of 12 genes, CDC20, TK1, KNL1, CENPE, STIL, ANLN, NDC1, NUF2, KIF20A, PLK4, CCNB1, and CCNA2
, and (ii) the quiescence state composed of 12 genes: COL5A1, TGFBI, TCEA2, WNT9A, MMP11, LAMB1, KRT14, LTBP1, PHLDB1, TIMP3, LRP1, and COL18A1.
Using miRGen and MicroCosm, we found that Ccna2
and Bcl10, two genes that were up-regulated in our mRNA arrays, were the predicted targets of the down-regulated rno-miR-489 and rno-let-7e*, respectively.
Symbol Gene name 1 CCNA1 Cyclin A1 2 CCNA2
Cyclin A2 3 CCNB1 Cyclin B1 4 CCND1 Cyclin D1 5 CCND2 Cyclin D2 6 CCND3 Cyclin D3 7 CCNE1 Cyclin E1 8 CCNE2 Cyclin E2 9 CCNH Cyclin H 10 CDC25A Cell division cycle 25 homolog A (S.