In studies to clarify epigenetic mechanisms in TGCTs, promoter methylation was shown in genes such as tumor suppressors APC, ARF, TP53, RARB2, BRCA1, RASSF1A, cell cycle regulator CCNA1
, DNA repair genes such as MGMT and hMLH1, transcription factor HOXA9, and PRSS21, which encodes the protein testisin in testis cell maturation (30).
qRT-PCR analysis confirmed that the mRNA levels of cyclin family members ( ccna1
, ccnb1 , ccnd1 , and cdk1 ), minichromosome maintenance proteins ( cdc45 , mcm2 , mcm3 , mcm4 , mcm5 , mcm6 , and mcm7 ), and proliferation cell nuclear antigen ( PCNA ) were significantly upregulated in ATF3-overexpressing CFs [Figure 3]a.
LINE-1, HS3ST2, CCNA1, EPB41L3, EDNRB, LMX1, and DPYS were hypermethylated in cervical cancer tissues, CIN III and CIN II, versus normal tissues and CIN I, of which EPB41L3 seems to be the best marker.
Methylated HS3ST2, CCNA1, EGFR promoter, FHIT, TFPI2, CpG6, and CpG15 sites were associated with HPV16 infection in the progression of cervical cancer .
Numerous pieces of evidence suggest that HPV16 E7 oncoprotein mediates DNA hypermethylation in the CCNA1 and CXCL14 promoter and suppresses gene expression.
In H1N1 infection, 4 DNBs (SEPT4, NPAT, STMN1, and CCNA1
) participate in cell cycle.
At the same time, the expression of seven genes (APC, CCNA1
, FASLG, FOS, GJA1, MAP2K6, and MID1) showed more than a 2-fold change.
Numerical labeling Gene names 1 CCNA1
2 CCNB1 3 CCND1 4 CCNE1 5 CDH1 6 CDT1 7 CDKN1B 8 CDK1 9 CDK2 10 CDK4 11 CDK6 12 TP53 13 GMNN 14 SKP2 Table 3: Determined system parameters.
The results showed that the decoction incubation reduced the levels of CCNA1, CCND1, CCND2, CCNH, CDKN1B, MCM2, ORC6L, and WEE1 and increased the levels of CDC7, CDK6, CDK7, E2F3, and RB1 (Table 1).
Moreover, we showed that the cell-cycle arrest was accompanied by down-regulation of CCNA1, CCND1, CCND2, CCNH, CDKN1B, MCM2, ORC6L, and WEE1 and upregulation of CDC7, CDK6, CDK7, E2F3, and RB1.
Symbol Gene name 1 CCNA1 Cyclin A1 2 CCNA2 Cyclin A2 3 CCNB1 Cyclin B1 4 CCND1 Cyclin D1 5 CCND2 Cyclin D2 6 CCND3 Cyclin D3 7 CCNE1 Cyclin E1 8 CCNE2 Cyclin E2 9 CCNH Cyclin H 10 CDC25A Cell division cycle 25 homolog A (S.
(310) Differentially expressed genes in ALK+ ALCL included genes encoding classical ALCL markers (ALK, CD30, MUC1 or EMA), immunoregulatory cytokines (IL-26, IL-31RA, IL-9, IL-1R2), Th17 cell-associated molecules (IL-17A, IL-17-F, ROR[gamma]), proliferation associated molecules (CCNA1
, AGT, PDE4DIP, UPK1B, CDC27), STAT3-regulated targets (SERPINB3, SERPINB4, SOCS1, SOCS3), genes identified in other tumors (RRAD, RAR[alpha], NRCAM, TMEM158, CA12), cytotoxic molecules (PRF1, GZMB), and immunosuppressive response molecules (LILRA3).