Methods: Meso scale discovery system and commercial ELISA kits were used to measure the concentrations of proinflammatory cytokines interleukin (IL)-1[sz]; tumor necrosis factor-alpha (TNF-a); IL-6; and IL-17 and CC and CXC chemokines CCL2, CCL4, CCL11, CCL13, CCL17, CCL22, and CCL26 and CXCL8, CXCL9, CXCL10, and CXCL11 in bronchoalveolar lavage fluid of asthmatics and controls.
Chemokines whose concentrations were measured in the present study included CXCL8 (IL-8), CXCL9 (monokine induced by gamma interferon), CXCL10 (interferon-gamma-induced protein 10 [IP-10]), CXCL11 (interferon-inducible T-cell alpha chemoattractant), CCL4 (macrophage inflammatory protein 1[sz] [MIP-1[sz]]), CCL11 (eotaxin-1), CCL26 (eotaxin-3), CCL17 (thymus and activation-regulated chemokine [TARC]), CCL22 (macrophage-derived chemokine [MDC]), CCL2 (monocyte chemoattractant protein 1 [MCP-1]), and CCL13 (MCP-4).
Meso scale discovery (MSD) system (MSD, Rockville, MD, USA)[sup] was used to measure the concentrations of proinflammatory cytokines and chemokines for IL-1[sz], IL-6, IL-17, IL-18, TNF-a, CXCL8, CXCL10, CCL2, CCL4, CCL11, CCL13, CCL17, CCL22, and CCL26 using a commercial kit (K15054G-2).
Concentrations of CC chemokines CCL2, CCL4, CCL11, CCL13, CCL17, CCL22, and CCL26 in bronchoalveolar lavage fluid
The median concentrations CCL4 (MIP-1[sz]), CCL11 (eotaxin-1), and CCL17 (TARC), but not CCL2 (MCP-1), CCL13 (MCP-4), CCL22 (MDC), and CCL26 (eotaxin-3), were significantly greater in BALF from total asthmatics compared with controls (CCL4: P = 0.