Nevertheless, it is still too early to end hope for CB1R inhibition in obesity treatment.
Rimonabant is an antagonist/inverse agonist for CB1R. Whether rimonabant restores the effects of HFD on body weight and glucose homeostasis as an antagonist or as an inverse agonist is controversial.
A clinical trial assessing the safety and efficacy of the CB1R inverse agonist taranabant in obese and overweight patients: low-dose study.
With all these antecedents in mind, the aim of this study was to evaluate the effects of oral administration of AEA to male mice pups during lactation on the abundance and binding capacity of the hypothalamic CB1R, to relate it with acute AEA-stimulated and long-term unstimulated food intake.
Rabbit polyclonal antibody for CB1R was used as the primary antibody (Cayman chemical Co, USA) and as the secondary antibody, an enzyme-conjugated anti-rabbit antibody was used (Bio-Rad, USA).
Experiment 3: Effects of CB1R and CB2R Signaling in Propofol-Induced Cardioprotection against I/R Injury.
Real-Time PCR for CB1R, CB2R, and FAAH mRNA Levels of Detection.
"The study shows that one of the most common effects of cannabinoid intoxication is due to activation of astroglial CB1Rs," the researchers wrote.
The researchers hope to explore the activities of endogenous endocannabinoids, which naturally trigger CB1Rs, on astroglial and other cells.
In contrast, animals lacking CB1Rs
in neurons still suffer the usual lapses.