CAT Assay

Chloramphenicol Acetyl Transferase Assay. A technique used to determine whether a given segment of DNA has an attached promoter, which would up-regulate transcription
References in periodicals archive ?
Parameters of the CAT assay included time to peak thrombin generation (PTm), endogenous thrombin potential, peak thrombin generation and velocity index (VIx).
By these modifications of the plasma CAT assay, we acceptably reduced quenching and issues related to unequal distribution of red blood cells.
This aspect, coupled with the radioactivity involved in the CAT assay, taints the CAT gene's usefulness as a reporter gene, says Keith V.
Gorman developed the CAT assay in 1980 as a postdoctoral fellow in the LMB/NCI.
The CAT assay has been evaluated in studies including comparisons with the BQ procedure, but not in multicenter evaluations.
LDL prepared by ultracentrifugation was measured by the CAT assay and revealed a mean recovery for LDL of 95% in 31 samples (108).
The very limited evaluation suggests that the CAT assay seems to be relatively specific for LDL-C but that some VLDL-C is measured as LDL-C, indicating interference with high TGs.