The effects of gemcitabine and combination of FA and gemcitabine on expressions of genes are important in apoptosis including CASP3, CASP7, CASP8, CASP9, BCL2, BAX, FAS, CYCS, TNF and PPARG, and were determined using qPCR analysis, after total RNA isolation and cDNA synthesis from control and dose group cells.
After the treatment with a combination of FA and gemcitabine, significant increases in the expression of CASP3, CASP7, CASP8, FAS, CYCS, TNF and PPARG were observed as 13.55, 9.48, 23.83, 51.63, 10.34, 17.86 and 14.63 folds, respectively compared with the control group (p<0.05).
Rueff, "Genetic susceptibility in acute pancreatitis: genotyping of GSTM1, GSTT1, GSTP1, CASP7
, CASP8, CASP9, CASP10, LTA, TNFRSF1B, and TP53 gene variants," Pancreas, vol.
In addition, the expression of proapoptotic caspases (CASP3, CASP7
, CASP8, and CASP9) is increased, while the expression of [Bcl.sub.2] (an antiapoptotic factor) is decreased in obesity .
The relationships of EPOR (erythropoietin receptor), MAPK14 (mitogen-activated protein kinase 1), BCL2L1 (BCL2-like1), KRT18 (keratin 18), PTPN6 (protein tyrosine phosphatase nonreceptor 6), CASP3 (caspase-3), TGFBR2 (transforming growth factor-beta, TGF[beta], type II receptor), AR (adrenergic receptor), and CASP7
(caspase-7) with gastric cancer were already known.
We used I-TASSER as the molecular modeling program because it was ranked as the best server for protein structure prediction in recent CASP7
, CASP8, and CASP9 experiments [21, 23, 24].
Furthermore, we have observed the gene expression pattern of farnesyl pyrophosphate synthase (FDPS), farnesyl-diphosphate farnesyl transferase 1 (FDFT1), caspase 7 (CASP7
), Rho family GTPase (RND3), Ras oncogene family member (RAB11A), and DNA methyl transferase 1 (DNMT1) in ALN treated and control Huh-7 cells.
TABLA III PATRON DE LATENCIA DEL VIRUS EPSTEIN BARR Y NEOPLASIAS ASOCIADAS Latencia Genes virales Neoplasias asociadas expresados I EBNA-1 Linfoma de Burkit EBERs BARF II EBNA-1 Linfoma de Hodgkin EBERs Cancer nasofaringeo LMP-1 Linfoma periferico LMP-2 T/NK BARF III Todos EBNAs Linfomas asociados a EBERs SIDA LMP-1 Desordenes LMP-2 linfoproliferativos BARF Postransplantes TABLA IV GENES CANDIDATOS Y LINFOMAS FAMILIARES Via Genes Ciclo celular/ AICDA, BAX, BCL2,BCL2A1,BCL2L1,BCL2L2, BCL2L10, Apoptosis BCL2L11(BIM), BCL6, BCL7A, BCL7C, BCL10, CASP1, CASP14, CASP4, CASP5, CASP6, CASP7
, CASP8AP2, CASP9, CCND1, LMO2, MYC, PIM1, RIPK1, RIPK2, TP53, TP53I.
Effector caspases (CASP3, CASP6, CASP7
) in turn cleave other protein substrates within the cell, to trigger the apoptotic process.
Zhang, "Template-based modeling and free modeling by I TASSER in CASP7
," Proteins, vol.69, no.S8, pp.108-117,2007.
The down-regulated genes were TRAIL, TNFR, AP13, IGFBP3, SARP3, PKB, IGFBP, CASP7
, CASP9, TNFSF6, TRICK2A, CAS, TRAIL-R2, RATS1, hTRIP, TNFb and TNFRSF5.