CCNDI, CDK6, p21, p53, Caspase-3 (CASP-3), Caspase-8 (CASP-8, Caspase-9 (CASP9), BCL-2, Ataxia Telangiectasia Mutated (ATM), Ataxia Telangiectasia and Rad-3 Related (ATP), BAX, Checkpoint kinase 1-2 (CHEK1, CHEK2) and Excision repair cross-complementing I (EPCCI) gene expression analyses were performed using the StepOnePlus quantitative RTPCR system (Applied-Biosystems, USA) with respect to SYBR Green (Thermo-Scientific, USA) method.
We determined that ISO has an effect on protease activities of CASP-3 and CASP-8.
We studied activated CASP-3 for exploring the mechanism of ISO-induced apoptosis in HT-29 cells.
LG exposure (LG) did not affect gene expression of IL-6, ICAM-1, and COX2 as well Casp-3, Casp-9, and CHOP.
In our study, exposure to LPS increases expression of both Casp-3 and Casp-9 as well as that of CHOP, while 1-hour pretreatment with LG blunted all proapoptotic factors to levels even lower than those observed in basal experimental conditions.
Caption: Figure 7: Effects of 0.7 mg/mL LG pretreatment on EPCs exposed for 23 hours to 10 ug/mL LPS: quantitative real-time RT-PCR analysis of Casp-3, Casp-9, and CHOP gene expression.