Effect of 1TAC on Neuro-pathological Evaluation of Hippocampal CA3
Hematoxylin and Erosin Staining: The distribution of pyramidal neuronal cells in hippocampal CA3
regions were examined under low (X10) power objective.
As seen from the spectra, the intensity of the bands at 3,200-3,500 [cm.sup.-1] and 1,567 [cm.sup.-1] relatively increases with increasing the grafting percent from CAI to CA3
. This is probably due to the increases of -NH groups, quinoid, and benzenoid units in the Ch-g-PANI copolymers.
Information processed by the dentate gyrus gets passed along to the CA3
, and then on to CA1.
In adults, mossy fibers normally project to proximal apical dendrites of pyramidal cells in the stratum lucidum (SL) of the CA3
(Figure 1(a)), whereas, during postnatal development, mossy fibers from the immature granule cells extend to both the SL and stratum oriens (SO), forming both suprapyramidal bundles (SPB) and infrapyramidal bundles (IPB), respectively (Figure 1(b)).
Every fifth section (1-in-5 series) containing the dorsal hippocampal CA3
subregion was collected (in PBS 0.1 M).
Patch-clamp whole-cell recordings were performed in CA3
PND 40 rats supplemented prenatally with either choline or DHA alone shows significantly (* p < 0.05) increased number of neural cells in the CA3
region of hippocampus when compared to the same in age matched normal control and saline control group of rats.
These layers sent outputs to dorsal and ventral dentate gyrus (DG, 2 layers, dorsal and ventral, size 20 x 40 each), CA3
(2 layers size 16 x 16 each), and CA1 (4 layers size 20 x 20 each, Table 1).
In the control group, pyramidal cells in the CA1, CA3
and CA4 regions were densely arranged, and had longer processes and were deeply stained.
activity was statistically significant between groups in the CA1 area of the ventral hippocampus and CA3
area, showing higher C.O.
In addition, Simvastatin (10, 20, and 40mg/kg) significantly reduced the total number of abnormal pyramidal cells in CA1 and CA3
hippocampal regions compared to the picrotoxin-alone group.
An unexpected and key new observation from this study was a substantial and significant increase in cell count number in the dentate gyrus region and specifically CA3