is a protein found on the surface of many different types of cells.
A genetic mutation in the protein c-kit
tyrosine kinase receptor--called a c-kit
oncogene--is found in 25 to 30 percent of tumors.
(6) showed that, in a transplantation model of murine [CALM/AF10.sup.+]leukemia, the myeloid leukemia cells were frequently [B220.sup.+], with clonal B cell receptor (BCR) rearrangement and lacked c-Kit
expression, demonstrating that these AML cells had lymphoid characteristics.
Mutational analysis for c-kit
(cd117) and PDGFRA was not detected, and there was also no mutation found on the BRAF gene.
Although with this kit 5 markers were provided, we could manage to analyze only 3 loci: BAT25 (c-kit
), D5S346 (APC) and D17S250 (hMSH2).
A clinical trial started in 2015 to test a type of stem cell in the heart called c-kit
cells, but it was called a halt in October 29 after Harvard Medical School retracted 31 papers upon which the trial was based.
Hematoxylin and eosin staining and immunohistochemical markers, such as c-Kit
, CD34, and Ki-67, were used to diagnose GISTs (Figure 1).
Harvard's investigation, however, found that so-called "c-kit
" stem cells do not function in the way Anversa described; follow-on studies have raised doubt that c-kit
cells can regenerate heart tissue.
In 15% of the cases, mutations are found in Exon 9 of the c-KIT
gene encoding the extracellular domain.
The researchers looked at Spred1 protein because it binds to c-Kit
, a molecule involved in signaling processes that govern HSC development and regulation.
It shows CD34 (+), vimentin (+), progesterone (+), S100 (-) and C-kit
(-) staining patterns, immunohistochemically.