2014), but only 5 of them include the genus Frieseomelita, in which only conventonal techniques (Giemsa staining, C-banding
, and base-specifc fuorochrome staining), have been applied (Rocha et al.
of BST and BT was highly heterochromatic and easily distinguished from A chromosomes (As) following the conventional C-banding
methodology, whereas heterochromatin can be observed in a more condensed or dispersed state along both Bs and As.
Constitutive heterochromatin regions were observed on the centromeres of chromosomes in all four species by using C-banding
. Nucleolus organizer regions (NOR) were observed on the short arms of two pairs of chromosomes.
acrobata female, including chromosome composition, C-banding
and NOR regions.
The distribution of constitutive heterochromatin (C-banding
) in this specimen showed pericentromeric bands in all autosomes.
proximus (CASTELNAU, 1855), based on conventional staining, C-banding
and Ag-NOR staining.
In this study, the molecular cytogenetic characterization of mitotic chromosomes of the Provence flat oyster or dwarf oyster, Ostrea stentina, was performed through Giemsa staining, chromosome measurements, in situ restriction endonuclease banding, C-banding
, fluorescence in situ hybridization with major ribosomal RNA genes (ITS1), and telomeric sequence [(TTAGGG).sub.n].
Mapping and organization of highly-repeated DNA sequences by means of simultaneous and sequential FISH and C-banding
Slides for C-banding
were treated according to the technique described by Sumner (1972) with minor modifications.
pattern seems to be characteristic of spiders.
In each generation, selection for translocation heterozygotes and against disassembled chromosomes MA1 was by C-banding
. Following BE4, translocation heterozygotes were self pollinated and their progenies screened by C-banding
to isolate translocation homozygotes.
The Andean specimens present C-heterochromatic blocks in most of their 22 chromosomes, whereas non-Andean specimens have only 4-7 autosomes with C-banding
. These heterochromatin differences are the likely cause of a striking DNA content variation (approximately 30%) between Andean and non-Andean insects.