Ethyl, propyl, and butyl esters
with the corresponding alcohols can be obtained in high yields in in situ alcoholysis of soybean oil from soybeans .
Product ion scans were produced by focusing M51 on the molecular masses [(M + H).sup.+] of the butyl ester of octanoylcarnitine (m/z 344) and its internal calibrator [[sup.2][H.sub.3]]octanoylcarnitine (m/z 347), while MS2 was used to scan fragment ions between m/z 50 and m/z 400.
Analysis of acylcarnitines after derivatization as butyl esters was recently proposed .
Tuning of the instrument was optimized with a solution containing deuterium-labeled calibrators prepared as butyl esters as described previously .
The fragmentation pattern of the protonated molecular ions, [[M + H].sup.+] of the butyl esters of octanoylcarnitine and [[sup.2][H.sub.3]] octanoylcarnitine, are shown in Fig.
We report the validation of a high-throughput method for the analysis of acylcarnitine butyl esters in infants with these disorders and include the results from a prospective study of the occurrence of PA and MMA in nearly 1 million newborns screened using MS/MS.
Samples were prepared and analyzed for acylcarnitines as butyl esters by electrospray MS/MS as described recently (12, 18).
The product ion spectra for the butyl esters of C3 and its internal standard, [[sup.2.H.sub.3]]C3, showed prominent ions at m/z 85, whereas the precursor ion spectra at m/z 85 showed a single ion at m/z 274 and m/z 277 for the protonated molecular ions of C3 and [[sup.2.H.sub.3]]C3, respectively (data not shown).