also controls cell growing, and interacts with RAD51 in DNA repair and homologous recombination (10).
7654dupA is predicted to result in the substitution of isoleucine for asparagine at amino acid position 2552 followed by a premature truncation of the BRCA2 protein
at amino acid position 2553.
The BRCA2 protein
is involved in repairing damaged DNA, playing a critical role in stabilising cells.
The host laboratory has purified full-length BRCA2 protein
and shown that it facilitates RAD51-mediated HR by acting as a molecular chaperone for RAD51 filament formation.
These observations have created a question: Why does the BRC4 peptide inhibit filament formation but the whole BRCA2 protein
appears to promote filament formation?
acts as a mediator, helping another protein, RAD51, to associate with a single strand of DNA and stimulating its activity.
The BRCA1 and BRCA2 protein
products are classified as tumour suppressor genes and are involved in DNA repair, genomic stability, transcriptional regulation and cell cycle control.
The specificity of the antibody was verified against the full-length (390 kDa) BRCA2 protein
extracted from HeLa cell nuclear extract.
The research studied how the BRCA2 protein
molecule interacts with Rad51 and how the process goes wrong when BRCA2 is faulty.
The functions of the BRCA2 protein
appear similar to that of BRCA1, although BRCA2 tumours have different cellular expression.
The mRNA is then translated to the BRCA1 and BRCA2 proteins
, which become products of nature found within the cell.
Non-cancerous cells will survive the treatment, because the normal BRCA1 and BRCA2 proteins
are key components in DNA repair.