The BRCA2 protein
is involved in repairing damaged DNA, playing a critical role in stabilising cells.
The host laboratory has purified full-length BRCA2 protein
and shown that it facilitates RAD51-mediated HR by acting as a molecular chaperone for RAD51 filament formation.
acts as a mediator, helping another protein, RAD51, to associate with a single strand of DNA and stimulating its activity.
The BRCA1 and BRCA2 protein
products are classified as tumour suppressor genes and are involved in DNA repair, genomic stability, transcriptional regulation and cell cycle control.
The specificity of the antibody was verified against the full-length (390 kDa) BRCA2 protein
extracted from HeLa cell nuclear extract.
The research studied how the BRCA2 protein
molecule interacts with Rad51 and how the process goes wrong when BRCA2 is faulty.
The functions of the BRCA2 protein
appear similar to that of BRCA1, although BRCA2 tumours have different cellular expression.
Non-cancerous cells will survive the treatment, because the normal BRCA1 and BRCA2 proteins
are key components in DNA repair.
The understanding of the functional role of BRCA1 and BRCA2 proteins
has led to the development of trials of poly(ADP-ribose) polymerase (PARP) inhibitors and platinum- versus taxane-based chemotherapy in patients with BRCA1/2-related metastatic breast cancer .
honed in on a protein, called the RAD51 protein, that's located near the same place on the gene as the BRAC1 and BRCA2 proteins