In both Boyden chamber
and wound-healing assays, we observed increased cell migration after treatment of SKBR3 cells with one of three OH-PBDEs (4'-OH-BDE-049, 5'-OH-BDE-099, and 3'-OH-BDE-154), and we found that pretreatment with G15 resulted in significant reduction of this cell migration, suggesting that it was mediated through activation of the GPER pathway.
Caption: Figure 3: Effect of IL-27 (50 ng/mL) and sorafenib (2 [micro]M) alone or combination on HTB-9 cell migration (A) and invasion (B), assessed by modified Boyden chamber
. Cells cultured with blank medium acted as control.
Based on the scratch assays results, we further investigated the effects of HGF incorporated into collagen and fibrin using a modified Boyden chamber
To this end, we used modified Boyden chambers
and agarose spot assays to assess the chemotattractant potential of these molecules on OEMSCs.