Immunohistochemical assays in prostatic biopsies processed in Bouin's fixative
. j Clin Pathol.
At the end of experiment, mice of each group were weighed, anaesthetized and processed for recovery of kidneys with subsequent fixation using Bouin's fixative
. Morphometric analysis showed an overall significant (p<0.05) reduction in body weight of mice of group DI(23.2g) and DII(20.8g) as compared to group C(29g).
After counting the implantation sites, the fetuses were recovered from the uteri, fixed in Bouin's fixative
for 48 h and then preserved in 70% alcohol for morphological and morphometric studies (Arshad et al., 2017).
Animals were sacrificed on the sixteenth day and testes were removed under anesthesia, cut into two pieces each and kept in Bouin's fixative
for 48 hours.
Sampled testes were immediately immersed in Bouin's fixative
and submitted to prefixation for approximately 20 minutes.
Fifteenth days after the start of the study, in sterile conditions, the left and right testes were removed by creating a gap in the lower abdomen, and the left testis was placed in Bouin's fixative
solution for histological examination.
hermafroditica, Bouin's fixative
penetrated tissue in less than 48 h, but, due to the high concentration of formalin (Bouin weak: 15% and strong: >20%), the specimens hardened and deteriorated at cellular level.
Two cover slips from each set of traps were preserved with modified Bouin's fixative
at a final concentration of 5% (Coats and Heinbokel, 1982), and four cover slips from each set were placed in small Petri dishes filled with water from the collection site for observation of live organisms with an inverted microscope (Invertscope, 25x magnification; Zeiss Corp.).
Whole fish were fixed in Bouin's fixative
and embedded in paraffin, and the gonad region was sectioned to 4 [micro]m, stained, and analyzed by light microscopy.
The implantation sites were counted and fetuses were dissected out of the uterus and then fixed in Bouin's fixative
for 48 hours.
For light microscopy, the specimens were fixed in Bouin's fixative
solution, processed routinely and the sections were stained with hematoxylin-eosin and Azo-Carmin to identify nerve fibers.
Gonads (from frogs at 1 month postmetamorphosis) attached to kidneys were fixed with Bouin's fixative
. For examining histologic structure, fixed gonads were then embedded in paraffin, sectioned at 6 [micro]m, and stained with hematoxylin and eosin.