Molecular identification of Borrelia crocidurae in a patient returning from Senegal.
Tick-borne relapsing fever imported from West Africa: diagnosis by quantitative buffy coat analysis and in vitro culture of Borrelia crocidurae. J Clin Microbiol.
Endemic foci of the tick-borne relapsing fever spirochete Borrelia crocidurae
in Mali, West Africa, and the potential for human infection.
In preliminary studies, we detected the DNA of Borrelia crocidurae
, Rickettsia spp., Tropheryma whipplei, and Coxiella burnetii in environmental samples and blood specimens from febrile patients in Senegal (3-6).
The spirochete Borrelia crocidurae causes erythrocyte rosetting during relapsing fever.
Epidemiologic, clinical, and laboratory findings and treatment for patients with Borrelia crocidurae meningoencephalitis * Variables Patient 1 Patient 2 Demographic factor Age, y/sex 36/M 57/M Country of origin/of Senegal/France France/France residence Travel country Senegal Senegal Travel dates 2009 Mar-May 2010 May Travel duration, d 53 15 Travel accommodation Family house Hotel Arthropod or insect bite No Yes report Individual vector No No First suspected diagnosis Malaria/quinine Sinusitis ([dagger])/ presumptive treatment Symptoms Oral temperature Yes Yes >38.5[degrees]C Chills No No Total no.
Positive control reactions for each assay incorporated DNA extracted from Borrelia crocidurae
. We injected the solution of Borrelia spp.
Approximately 20 travel-related cases have been published in the past 25 years, and most of them have been acquired in western Africa (mainly Senegal), where Borrelia crocidurae
is the predominant species (3).
infection of Ornithodoros erraticus (Lucas, 1849) ticks in Tunisia.
DNA sequencing demonstrated that Borrelia crocidurae
A comparative study of three methods of detection of Borrelia crocidurae
in wild rodents in Senegal.
was the only species identified.