STRAINS AVAILABLE AS ASSAYED CONTROLS * Adenovirus * Parainfluenza 1 * Parainfluenza 2 * Parainfluenzal * Recombinant Parainfluenza 4 * Recombinant Rhinovirus * Bordetella pertussis * Bordetella holmesii
* Influenza A (H1N1) * Influenza A (H3N2) * Influenza B * RSV A * RSV B * Bordetella parapertussis * Coronavirus * Rotavirus SA11 * NorovirusGWA * Bordetella parapertussis * Chlamydia pneumoniae * Mycoplasma pneumoniae * Chlamydia trachomatis * Neisseria gonorrhoeae * T.
: still emerging and elusive 20 years on.
Real-time PCR assay targeting IS481 of Bordetella pertussis and molecular basis for detecting Bordetella holmesii
. J Clin Microbial 2001;39(5):19631966.
pertussis, 11 (5.7%) for Bordetella parapertussis, two (1.0%) for Bordetella holmesii
, and five (2.6%) were indeterminate.
The target for the Bordetella pertussis PCR reaction is also found in Bordetella holmesii
. A false-positive result for Bordetella pertussis DNA may occur if Bordetella holmesii
is present in the sample; however, Bordetella holmesii
is found at a much lower prevalence than Bordetella pertussis.
pertussis and Bordetella holmesii
and may be present in Bordetella bronchiseptica (6-10) (see Table 1 in the Data Supplement that accompanies the online version of this Technical Brief at http://www.clinchem.org/content/vol51/issue12).
(22) The TaqMan primers and probes presented in this study also exhibit cross-reactivity with Bordetella holmesii
but fail to produce amplicon from B parapertussis, Bordetella bronchispetica, Haemophilus influenza, Moraxilla catarrhalis, Legionella pneumophila, or Streptococcus pneumoniae in high input as anticipated (E.L.C., P.P., and N.A., unpublished data, 2001).
To the Editor: We report a case of recurrent Bordetella holmesii
bacteremia with 4 clinical manifestations: 3 episodes of cellulitis and 1 episode of pneumonia.
PCR testing yielded two specimens with results consistent with Bordetella holmesii
On the basis of bacterial 16S rRNA gene sequence analysis, an emerging pathogen, Bordetella holmesii
, has been successfully identified in the immunocompromised hosts [130, 131].
Subsequent biochemical analysis, 16S rRNA sequencing, and DNA relatedness studies revealed that these strains were new Bordetella species, which was named Bordetella holmesii
to honor Barry Holmes (10).
We describe the epidemiology of a pertussis outbreak in Japan in 2010-2011 and Bordetella holmesii